gov.noaa.nmfs.inport:39258 eng UTF8 dataset National Centers for Coastal Ocean Science resourceProvider NCCOS Scientific Data Coordinator NCCOS.data@noaa.gov pointOfContact 2025-05-15T19:15:54 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science bsdna_h 1992 publication NOAA/NMFS/EDM 39258 https://www.fisheries.noaa.gov/inport/item/39258 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Piniak, Greg greg.piniak@noaa.gov principalInvestigator https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx WWW:LINK-1.0-http--link Citation URL Online Resource download tableDigital In order to determine the current status of and to detect any long-term trends in the environmental quality of U.S. nearshore waters, NOAA initiated the National Status and Trends program in 1984 with its National Benthic Surveillance Project. The primary objective of the Benthic Surveillance Project was to quantify concentrations of a suite of organic and inorganic contaminants in the livers of fish and surficial sediments from selected sites in the coastal and estuarine waters of the United States. In addition, the levels of certain indicators of the biological effects of these contaminants were measured. Incidences of visible lesions, including fin erosion, have been noted and histopathological examinations of various tissues have been carried out. Originally histopathological examinations determined the prevalence of any identifiable disease conditions in samples of liver, kidney, and gill tissue.In addition, the Benthic Surveillance Project tested fish livers from 30 locations in 1991 to identify the concentrations of DNA xenobiotic adducts. DNA xenobiotic adducts are a bioindicator of exposure to genotoxic compounds. Measurement of biological markers such as DNA xenobiotic adducts have been used to improve the evaluation of contaminant exposure and responses in indigenous fish, and thus reinforce the connection between exposure to toxic chemicals and the observed injuries. In response to concerns over environmental quality of the Nation's coastal and estuarine ecosystems, NOAA created the National Status and Trends (NSandT) Program in 1984. From 1984 through 1993, the Benthic Surveillance Project monitored chemical concentrations in the livers (and for metabolites of PAH's in the bile) of bottom-dwelling fish and in sediments at the sites of fish capture. The Benthic Surveillance Project also measured the biological effects of contaminant exposure, primarily as prevalence's of toxicopathic liver diseases. completed NCCOS Scientific Data Coordinator NCCOS.data@noaa.gov pointOfContact NCCOS Scientific Data Coordinator NCCOS.data@noaa.gov custodian notPlanned OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF AMERICA place Global Change Master Directory (GCMD) Location Keywords 20.8 Coastal Monitoring DNA DNA abnormalities DNA adducts DNA xenobiotic adducts NCCOS Research Data Type > Field Observation NCCOS Research Priority > Long-term Monitoring NCCOS Research Priority > Stressors, Impacts, Mitigation, and Restoration (SIMR) NCCOS Research Topic > Bioeffects/Toxicity NCCOS Research Topic > Chemical Contaminants NCCOS Research Topic > Monitoring NSandT deoxyribonucleic acid genotoxic exposure oceans xenobiotic deoxyribonucleic acid adducts theme Baltimore Harbor, Brewerton Channel Boston Harbor, Deer Island Cape Elizabeth, Richmond Island Cape Fear River, Horseshoe Shaol Charlotte Harbor, Cape Haze Chesapeake Bay, Chester River Chesapeake Bay, James River Chesapeake Bay, Patuxent River Choctawhatchee Bay, Choctawhatchee Bay Dana Point Harbor, Outside Delaware Bay, Cherry Island Range Gulf of Mexico Johns Bay, Pemaquid Neck Lower Laguna Madre, Laguna Heights Lower Laguna Madre, Long Island NCCOS Research Location > Geographic Area > Coastal Ocean NCCOS Research Location > Region > Alaska NCCOS Research Location > Region > East Coast NCCOS Research Location > Region > Gulf of Mexico NCCOS Research Location > Region > West Coast NCCOS Research Location > U.S. States and Territories > Alabama NCCOS Research Location > U.S. States and Territories > Alaska NCCOS Research Location > U.S. States and Territories > California NCCOS Research Location > U.S. States and Territories > Connecticut NCCOS Research Location > U.S. States and Territories > Delaware NCCOS Research Location > U.S. States and Territories > Florida NCCOS Research Location > U.S. States and Territories > Georgia NCCOS Research Location > U.S. States and Territories > Louisiana NCCOS Research Location > U.S. States and Territories > Maine NCCOS Research Location > U.S. States and Territories > Maryland NCCOS Research Location > U.S. States and Territories > Massachusetts NCCOS Research Location > U.S. States and Territories > Mississippi NCCOS Research Location > U.S. States and Territories > New Jersey NCCOS Research Location > U.S. States and Territories > New York NCCOS Research Location > U.S. States and Territories > North Carolina NCCOS Research Location > U.S. States and Territories > Oregon NCCOS Research Location > U.S. States and Territories > Rhode Island NCCOS Research Location > U.S. States and Territories > South Carolina NCCOS Research Location > U.S. States and Territories > Texas NCCOS Research Location > U.S. States and Territories > Virginia NCCOS Research Location > U.S. States and Territories > Washington New Bedford Harbor, Clarks Point Pascagoula River, Escatawpa River Pensacola Bay, Pensacola Bay Raritan Bay, East Reach Raritan Bay, Upper Bay Salem Harbor, Folger Point San Antonio Bay, Mosquito Point San Diego Bay, North San Diego Bay, Twenty Eighth Street San Francisco Bay, Hunters Point San Francisco Bay, Southampton Shoal San Pedro Bay, Outer Harbor Sapelo Sound, Dog Hammock St. Andrews Bay, Military Point St. Johns River, Arlington Channel St. Lucie River, Stuart U.S. Exclusive Economic Zone place DOC/NOAA/NOS/NCCOS > National Centers for Coastal Ocean Science, National Ocean Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian NSandT project InPort otherRestrictions Cite As: National Centers for Coastal Ocean Science, [Date of Access]: National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science [Data Date Range], https://www.fisheries.noaa.gov/inport/item/39258. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: None otherRestrictions Use Constraints: NOAA requests that all individuals who download NSandT data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by NOAA through its National Status and Trends Program". otherRestrictions Distribution Liability: None unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 39258 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nos/nccos/dmp/pdf/39258.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference vector eng; US environment Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 3; ESRI ArcCatalog 8.3.0.800 -166.5 -67.3 25.6 70.5 | Currentness: Publication Date 1991 false eng false Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data 2017-04-17 publication Text (Unstructured) XML (Structured Text) NCCOS Scientific Data Coordinator NCCOS.data@noaa.gov distributor https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx WWW:LINK-1.0-http--link https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx Live Data and Maps download https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx WWW:LINK-1.0-http--link https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx Live Data and Maps download dataset Horizontal Positional Accuracy The same kind of field/site data have been supplied since the start of the Benthic Surveillance Project, in 1984. Original site coordinates were derived from Loran-C time conversions. Early sites information resulting from Loran-C was converted from time delay information to latitudes and longitudes. These earlier data may be suspect when sites were located close to large structures that could have interfered with accurate time delays. When GPS was first available the signal was intentionally degrades so earlier coordinate information, even if it resulted from GPS, is not as accurate as data would be today. Because fish are not sessile, fish trawls have been made along different tracks in the water body of interest. The latitude/longitude coordinates provided in this file represent a nominal site center and trawling occurs within a 1 km radius of this location. Completeness Report The quality of the analytical data generated by the NSandT Program is overseen by the QA Project component, which has been in operation since 1985 and is designed to document sampling and analytical procedures, and to reduce intralaboratory and interlaboratory variation. The QA Project documentation will facilitate comparisons between different monitoring programs with similar QA activities and thus will extend the temporal and spatial scale of such programs. To document laboratory expertise, the QA Project requires all NSandT laboratories to participate in a continuing series of intercomparison exercises utilizing a variety of materials. The organic analytical intercomparison exercises are coordinated by the NIST, and the inorganic exercises by the National Research Council (NRC) of Canada. Details of quality assurance for the Benthic Surveillance Program can be found in Lauenstein and Cantillo, 1993. Conceptual Consistency The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. To assess the reproducibility of results every tissue sample was analyzed in duplicate. The primary collection apparatus was Otter trawls. Occasionally, fish were taken with hook and line, or with seine nets. These alternate collection methods were necessary because larger fish, such as older Atlantic croaker, were able to avoid an Otter trawl, or were found in untrawlable habitats such as shallow water, along marsh edges, and over oyster reefs. Fish liver tissue samples were analyzed for DNA-xenobiotic adducts in liver via Phosphorus 32-postlabeling assay to indicate genotoxic exposure. The analysis of DNA adducts by Phosphorus 32-postlabeling is a multistep process involving a series of biochemical reactions. First, DNA is hydrolyzed enzymatically to 3'monophosphates. The digest is then enriched in xenobiotic-modified mononucleotides by the selective removal of normal nucleotides. Following the enrichment step the adducted DNA is enzymatically labeled at the 5'-hydroxyl position with [Phosphorus 32] phosphate to form [5'-Phosphorus 32] deoxyribonucleoside 3',5'-bisphosphates. Separation of the Phosphorus 32-labeled adducts is usually accomplished by two-dimensional, thin-layer chromatography (TLC) on polyethyleneimine (PEI)-modified cellulose sheets. Autoradiography or storage phosphor imaging (Reichert et al., 1992) is then used to locate the radiolabeled adducts on the chromatogram (Reichert and French, 1994).The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. 1991-01-01T00:00:00