gov.noaa.nmfs.inport:28654 eng UTF8 dataset NMFS Office Of Sustainable Fisheries 1315 East-West Highway, 13th Floor Silver Spring MD 20910 resourceProvider Lynn, Shannara 228-549-1730 3209 Frederic Street Pascagoula MS 39567 shannara.lynn@noaa.gov pointOfContact 2024-02-29T00:00:00 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) Species ID Project Species ID publication NOAA/NMFS/EDM 28654 https://www.fisheries.noaa.gov/inport/item/28654 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information tableDigital Up to 10 individuals of a fin fish, shark, and crustacean species are collected and morphologically identified by Southeast Fisheries Science Center. Water-soluble sarcoplasmic proteins are extracted from the tissue; proteins are analyzed by microfluidic electrophoresis (Agilent Bioanalyzer 2100) to generate species-specific protein patterns. The protein patterns are entered into a database to determine which are the most abundant for each species. The proteins that are seen in all 10 individuals are transferred to the excel pattern matching library database that uses NSIL designed formulas to calculate the range of each protein (+/- 1.5 to 3%) and compare an unknown protein to pattern to those in the pattern matching library. The pattern matching library is designed to hold the highly abundant proteins extracted from tissue collected from 10 individuals of a species and compare an unknown pattern for identification. Shannara Lynn, Courtney Ford, Calvin Walker, Cheryl Lassiter underDevelopment Bell, Jon 1315 East-West Highway, 13th Floor Silver Spring MD 20910 jon.bell@noaa.gov pointOfContact Lassitter, Cheryl 228-762-7402 228-762-7144 3209 Frederic Street Pascagoula MS 39567 Cheryl.Lassitter@noaa.gov custodian continual Protein patterns Species ID theme DOC/NOAA/NMFS/OSF > Office of Sustainable Fisheries, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Species ID Project project InPort otherRestrictions Cite As: NMFS Office Of Sustainable Fisheries, [Date of Access]: Species ID Project [Data Date Range], https://www.fisheries.noaa.gov/inport/item/28654. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: The database is only accessible through the facility's network and protected from public access by a firewall. otherRestrictions Distribution Liability: All the proteins in the library are from authenticated species and the most abundant with-in 10 individuals. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 28654 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/osf/dmp/pdf/28654.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US oceans 2010 Microsoft Excel Most species come from all areas of the Gulf of Mexico. However, so samples are shipped from other areas such as Hawaii | Currentness: as samples arrive and are processed 2011 false eng false Spreadsheet Species Protein Repeatability 2018-03-23 publication CSV - Comma Separated Values (Text) Bell, Jon 1315 East-West Highway, 13th Floor Silver Spring MD 20910 jon.bell@noaa.gov distributor https://noaa-fisheries-hq.data.socrata.com/Seafood-Safety/Species-Protein-Repeatability/ub94-pbzc WWW:LINK-1.0-http--link Species ID download dataset Representativeness The number of proteins in the pattern matching library are species specific. Accuracy Individuals are authenticated morphologically by a fisheries biologist, documented and photographed for outside review. Analytical Accuracy Protein size can be affected by many differing environmental factors. Due to this variation, a protein size range (+/- 1.5 to 3%) is used. Quantitation Limits The number of proteins in the pattern matching library are species specific. Bias If the individual is misidentified (morphologically), the protein patterns can shift in the wrong direction or some high abundant protein can be excluded that should not. Comparability Each species in the library is compared to the library and others of that species not used as one of the to individuals is compared to verify the accuracy of the library. Completeness Measure The ideal number of species-specific proteins is greater than five. The highest amount of proteins extracted is 16 and then decreased to only include those observed in all 10 individuals. Precision Protein size can be affected by many differing environmental factors. Due to this variation, a protein size range (+/- 1.5 to 3%) is used. Analytical Precision Protein size can be affected by many differing environmental factors. Due to this variation, a protein size range (+/- 1.5 to 3%) is used. Field Precision On other analyses have been performed for comparison. Sensitivity The pattern matching library compares all proteins of an unknown to the species specific proteins with-in the range (+/- 1.5 to 3%). Detection Limit The height threshold is initially set to 5FU and raised incrementally for each individual to remove insignificant proteins until the total number of proteins is less than 16 Completeness Report Only proteins that are seen in all 10 individuals, and above the height threshold of 5FU are inserted into the pattern matching library. Conceptual Consistency Always. A specimen is caught and recorded on the vessel as its location, date and condition. After the specimen is brought to NSIL, it is identified by a fisheries biologist and the data recorded on the vessel is recorded into the database. The specimen is process and the number of vials is recorded.