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This project assesses a suite of analytical methods for predictive utility by evaluating broodstock and following the infection fate of their offspring. In addition to measuring fork length (mm) &amp; weight (g) of individual offspring, kidney tissue is analyzed for Renibacterium salmoninarum by enzyme-linked immunoassay (ELISA), fluorescent antibody microscopy, and duplex quantitative real-time polymerase chain reaction (qPCR). Individual fish data are recorded by spreadsheet. This project is conducted by Oregon Department of Fish &amp; Wildlife in collaboration with NOAA personnel. This is a self-contained project.

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