gov.noaa.nmfs.inport:18020 eng UTF8 dataset Spreadsheet Northwest Fisheries Science Center 206-860-3200 2725 Montlake Boulevard East Seattle WA 98112 USA https://www.nwfsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information resourceProvider Northwest Fisheries Science Center 206-860-3200 2725 Montlake Boulevard East Seattle WA 98112 USA nmfs.nwfsc.metadata@noaa.gov https://www.nwfsc.noaa.gov WWW:LINK-1.0-http--link NWFSC Home Website listed for Northwest Fisheries Science Center information pointOfContact 2026-03-04T19:17:17 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) Sablefish GI microbiome & histology - Incorporating Gastrointestinal Microbiome Analysis into Fish Nutritional Assessments Sablefish GI microbiome & histology (Incorporating Gastrointestinal Microbiome Analysis into Fish Nutritional Assessments) 2012-06-01 creation 2019-05-15 publication NOAA/NMFS/EDM 18020 https://www.fisheries.noaa.gov/inport/item/18020 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Rhodes, Linda D 206-860-3279 206-860-3467 2725 Montlake Blvd East Seattle WA 98112 Linda.Rhodes@noaa.gov originator https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/datasets/dataset/7390 WWW:LINK-1.0-http--link Sablefish GI microbiome & histology NWFSC Dataset Information page. Raw & refined classification & abundance data from array analysis & histology analysis download tableDigital Sustainable culture of sablefish (Anoplopoma fimbria) is an active research area at NWFSC, with a primary objective of rearing a human food source under healthy, economic, and sustainable conditions. Development of sustainable feed for juvenile fish through replacement of marine fish oils is key for economic and ecological viability of sablefish culture. This project intends to analyze the GI microbiome of juvenile sablefish, comparing three feeds (corn oil, linseed oil, BioOregon BioBrood) used in a National Marine Aquaculture Initiative (NMAI)-funded project examining the effects of substitute lipids on growth, production composition, and lipid bioconversion. Community DNA from GI mucus and luminal contents will be extracted, quantified, and quality checked. DNA will be submitted for analysis by a microbial phylogenetic microarray bearing over a million probes for classifying more than 59,000 bacterial taxa. Second Genome, a company with exclusive licensing for this phylogenetic microarray, will conduct the the analysis for classification, relative abundance, & ordination. Complementary to microbiome analysis, morphological changes in gastrointestinal tissues will be assessed for each fish by light microscopic histology (collaboration with Mark Myers). Although this is a stand-alone project, the objective is for the microbiome approach to become an integral component of finfish nutrition research. Raw & refined classification & abundance data from array analysis & histology analysis. Laboratory data completed Rhodes, Linda D 206-860-3279 206-860-3467 2725 Montlake Blvd East Seattle WA 98112 Linda.Rhodes@noaa.gov pointOfContact Rhodes, Linda D 206-860-3279 206-860-3467 2725 Montlake Blvd East Seattle WA 98112 Linda.Rhodes@noaa.gov custodian Alternative feeds aquaculture fish diet microbiome sablefish theme Montlake NWFSC Montlake place Instrument Not Applicable instrument Animal and Plant Collection Device platform DOC/NOAA/NMFS/NWFSC > Northwest Fisheries Science Center, National Marine Fisheries Service, NOAA. U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Migrated from Resource Enhancement and Utilization Technologies Division project InPort otherRestrictions Cite As: Northwest Fisheries Science Center, [Date of Access]: Sablefish GI microbiome & histology - Incorporating Gastrointestinal Microbiome Analysis into Fish Nutritional Assessments [Data Date Range], https://www.fisheries.noaa.gov/inport/item/18020. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: NA unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 18020 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nwfsc/dmp/pdf/18020.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US oceans Spreadsheet -122.3062 -122.2962 47.6449 47.6549 NWFSC Montlake: NWFSC Montlake molecular biology & histology labs 2012-05-01 2013-04-30 Subject to Public Access to Research Results (PARR): Yes Peer Reviewed Publication: Dietary effects on sablefish gastrointestinal microbiome & histology. Peer reviewed publication Presentation: Microbiome of Sablefish under Different Dietary Regimens. Aquaculture meeting presentation Report: Report to NOAA Aquaculture Program. Funding report false eng false Spreadsheet Comparative bacterial and archaeal gastrointestinal abundances from sablefish on different diets 2019-07-02 publication Northwest Fisheries Science Center 206-860-3200 2725 Montlake Boulevard East Seattle WA 98112 USA nmfs.nwfsc.metadata@noaa.gov https://www.nwfsc.noaa.gov WWW:LINK-1.0-http--link NWFSC Home Website listed for Northwest Fisheries Science Center information distributor https://www.webapps.nwfsc.noaa.gov/apex/parrdata/inventory/tables/table/comparative_bacterial_and_archaeal_gastrointestinal_abundances_from_sablefish_on_different_diets WWW:LINK-1.0-http--link Comparative bacterial and archaeal gastrointestina These data were derived from a feeding study with juvenile sablefish, comparing plant-based feeds (flax, corn) with fish-based feed (reference). Intestinal microbiomes were determined by hybridization of a gastronintesntial DNA microarray. download https://www.webapps.nwfsc.noaa.gov/apex/parr/comparative_bacterial_and_archaeal_gastrointestinal_abundances_from_sablefish_on_different_diets/data/page/ WWW:LINK-1.0-http--link Comparative bacterial and archaeal gastrointestina These data were derived from a feeding study with juvenile sablefish, comparing plant-based feeds (flax, corn) with fish-based feed (reference). Intestinal microbiomes were determined by hybridization of a gastronintesntial DNA microarray. download dataset Accuracy Medium sample signals are standardized before comparisons