gov.noaa.nmfs.inport:27478 eng UTF8 dataset MS Excel Spreadsheet Northeast Fisheries Science Center (508)495-2000 166 Water Street Woods Hole MA 02543-1026 USA https://www.nefsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 8:00 a.m. to 4:30 p.m. Monday - Friday resourceProvider Horodysky, Andrij 74 Magruder Road, Highlands NJ 07732 andrij.horodysky@noaa.gov pointOfContact 2025-10-08T21:40:38 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) Experimental tests of the effects of OA on early life history of marine fishes effects of OA on early life history of marine fishes publication NOAA/NMFS/EDM 27478 https://www.fisheries.noaa.gov/inport/item/27478 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Chambers, Robert Christopher 732-872-3075 732-872-3088 74 Magruder Road Highlands NJ 07732-0428 USA chris.chambers@noaa.gov originator tableDigital These data characterize treatments and outcomes in laboratory-based ocean acidification experiments conducted that the NOAA NEFSC Howard Laboratory. Experiments vary by species used, scope of study (experiment duration and species’ life-stages examined), and experimental design including number of treatments, levels, and replicates, and the suite of response variables. This experiment used summer flounder (Paralichthys dentatus) as subjects, one treatment (concentration of aqueous pCO2), three treatment levels (775, 1808, and 4714 uatm pCO2, pHs of 7.8, 7.5, and 7.1, respectively), three replicates (replicated downstream from each unique CO2 source), and the following response variables: survival of embryos to hatching and of larvae to age 28 d post-hatching; size, shape, and image-based developmental features of larvae; and histopathologically determined status of key organs (eye, heart, liver, gall bladder, gastro-intestinal tract, epidermis, kidney, spinal cord, and muscles) and cranio-facial and skeletal elements. Contribution to ocean acidification knowledge regarding biological responses to elevated CO2. Supported by NOAA’s Ocean Acidification Program and the Northeast Fisheries Science Center. completed Horodysky, Andrij 74 Magruder Road, Highlands NJ 07732 andrij.horodysky@noaa.gov pointOfContact Habeck, Ehren 74 Magruder Road Highlands NJ 07732 ehren.habeck@noaa.gov custodian notPlanned CO2 Paralichthys dentatus development embryos growth larvae ocean acidification summer flounder survival theme Mid-Atlantic USA place pelagic stratum DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Fisheries Ecology Branch (FEB) project InPort otherRestrictions Cite As: Northeast Fisheries Science Center, [Date of Access]: Experimental tests of the effects of OA on early life history of marine fishes [Data Date Range], https://www.fisheries.noaa.gov/inport/item/27478. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: NEFSC Data Access Constraint: If data are stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 27478 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nefsc/dmp/pdf/27478.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US oceans Data entered and saved in Microsoft Excel versions 2007, 2010. Data reside on network drive at location ‘R:\RESEARCH\OA - FY12\summer flounder’, and are backed up nightly on NEFSC Sandy Hook Lab servers. New Jersey, USA coastal waters (source of adults used to produce the offspring used in these experiments Experiments were conducted seasonally, following the availability of spawning products from the species and source populations. In this experiment, adults were collected during June – September 2011 and were spawned in December 2011. Embryos and larvae were maintained in the experiment through January 2012. Images from experiments were analyzed during 2012 and histopathology was performed during 2012-13. 2010 2014 Chambers, R.C., A.C. Candelmo, E.A. Habeck, M.E. Poach, D. Wieczorek, K.R. Cooper, C.E. Greenfield, and B.A. Phelan. 2014. Effects of elevated CO2 in the early life stages of summer flounder, Paralichthys dentatus, and potential consequences of ocean acidification. Biogeosciences, 11, 1613-1626, doi:10.5194/bg-11-1613-2014. false eng false Spreadsheet SumFL Egg Survival_DATA 2017-09-19 publication SumFL Egg Survival_TERMS 2017-09-19 publication SumFL Larval Cranio-facial_DATA 2017-09-19 publication SumFL Larval size_TERMS 2017-09-19 publication SumFL Larval size_DATA 2017-09-19 publication SumFL YSL Survival_DATA 2017-09-19 publication SumFL YSL Survival_TERMS 2017-09-19 publication SumFL Larval Cranio-facialTERMS 2017-09-19 publication Chambers, Robert Christopher 732-872-3075 732-872-3088 74 Magruder Road Highlands NJ 07732-0428 USA chris.chambers@noaa.gov distributor https://console.cloud.google.com/storage/browser/nmfs_odp_nefsc/PARR/EAD/FEB/27478 WWW:LINK-1.0-http--link https://console.cloud.google.com/storage/browser/nmfs_odp_nefsc/PARR/EAD/FEB/27478 4 Excel Files: 27478_EGG_SURVIVAL.xls 27478_LARVAL_CF.xls 27478_LARVAL_SIZE.xls 27478_YSL_SURVIVAL.xls download dataset Completeness Report Data provided are the individual-level observations prior to statistical reduction to means, principal components, etc. Data were not filtered or selected after check for errors. Data were collected by pre-planned design and varied with experiment. Data on embryo deaths and hatchlings were collected at a daily frequency; size and shape data of hatchlings (Nmax = 10 per replicate) were collected from the interquartile range of the hatch frequency distribution once hatching commenced; mortality of larvae was checked daily; larvae were sampled for image-based size and shape every 7 days through 28 days post-hatching. Data from images were scored via image analysis software (Image Tool v.3). Histopathology data were collected from preserved larvae (staining sequence in Chambers et al. 2014 ibid.). All data were entered into Excel spreadsheets.