gov.noaa.nmfs.inport:26979 eng UTF8 dataset MS Excel Spreadsheet Northeast Fisheries Science Center (508)495-2000 166 Water Street Woods Hole MA 02543-1026 USA https://www.nefsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 8:00 a.m. to 4:30 p.m. Monday - Friday resourceProvider Kapareiko, Diane N 203-882-6539 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA diane.kapareiko@noaa.gov pointOfContact Wikfors, Gary H 203-882-6525 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA gary.wikfors@noaa.gov pointOfContact 2026-03-04T19:17:17 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) Oyster Larval Survival Counts from Probiotic OY15 Experiments Probiotic OY15 larval survival counts 2011 publication NOAA/NMFS/EDM 26979 https://www.fisheries.noaa.gov/inport/item/26979 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Kapareiko, Diane N 203-882-6539 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA diane.kapareiko@noaa.gov originator Wikfors, Gary H 203-882-6525 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA gary.wikfors@noaa.gov originator http://www.bioone.org/doi/full/10.2983/035.030.0303 WWW:LINK-1.0-http--link Citation URL ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: I. ISOLATION AND SCREENING FOR BIOACTIVITY. Journal of Shellfish Research, Vol. 30, No. 3, 609–615, 2011 download http://www.bioone.org/doi/full/10.2983/035.030.0304 WWW:LINK-1.0-http--link Citation URL ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: II. EFFECTS OF A VIBRIO SP. PROBIOTIC CANDIDATE UPON SURVIVAL OF OYSTER LARVAE (CRASSOSTREA VIRGINICA) IN PILOT-SCALE TRIALS. Journal of Shellfish Research, Vol. 30, No. 3, 617–625, 2011. download tableDigital Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. Probiotic candidate selection was based upon the Kirby Bauer Disc Diffusion Method, in which zones of inhibition suggested competitive exclusion of a known shellfish larvae pathogen (B183). In challenge studies, survival of 2-day old oyster larvae supplemented with probiotic candidate OY15 (Vibrio sp.) was similar (p<0.3883) to that of control larvae (no added bacteria), indicating no harmful effects. Further, addition of OY15 to oyster larvae challenged with B183 significantly improved survival (p<0.0141) compared to the pathogen alone. An in vitro study to determine the effects of OY15 or the pathogen B183 upon oyster hemocytes and their immune functions demonstrated immuno-stimulation of oyster hemocytes by OY15 and immune-suppression by B183, suggesting an immuno-modulation mechanism for OY15. Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. completed Wikfors, Gary H 203-882-6525 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA gary.wikfors@noaa.gov pointOfContact Kapareiko, Diane N 203-882-6539 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA diane.kapareiko@noaa.gov pointOfContact Kapareiko, Diane N 203-882-6539 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA diane.kapareiko@noaa.gov custodian Wikfors, Gary H 203-882-6525 203-882-6570 212 Rogers Avenue Milford CT 06460-6499 USA gary.wikfors@noaa.gov custodian asNeeded Crassostrea virginica Vibrio disease prevention larvae survival oyster larviculture probiotic bacteria shellfish larvae theme spring temporal Northeastern United States place Shellfish hatcheries stratum DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Aquaculture Sustainability Branch (ASB) project InPort otherRestrictions Cite As: Northeast Fisheries Science Center, [Date of Access]: Oyster Larval Survival Counts from Probiotic OY15 Experiments [Data Date Range], https://www.fisheries.noaa.gov/inport/item/26979. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: The Milford laboratory is currently involved in a CRADA (Cooperative Research and Development Agreement) with Envera LLC, to mass-culture Milford probiotic strain OY15 and make freeze-dried or spray-dried formulations. This may cause a constraint on access to the data as intellectual property, until the CRADA has been completed (June 2016) and agreed upon with collaborators. NEFSC Data Access Constraint: If data is stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 26979 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nefsc/dmp/pdf/26979.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US oceans Microsoft Excel 2010, Windows 7 Professional, summer 2013 oyster larvae challenges.xls, 33kb -73.053572 -73.043572 41.212161 41.222161 Oysters cultured, bled or spawned occurred at the Milford Laboratory with the above latitude and longitude. Bacterial culturing of digestive glands of oysters began 2006 resulting in 2011 publications. 2006 2011 ISOLATION AND EVALUATION OF NEW PROBIOTIC BACTERIA FOR USE IN SHELLFISH HATCHERIES: I. ISOLATION AND SCREENING FOR BIOACTIVITY. H. J. LIM, D. KAPAREIKO, E. J. SCHOTT, A. HANIF AND G. H. WIKFORS. Journal of Shellfish Research, Vol. 30, No. 3, 609–615, 2011. false eng false Spreadsheet Spreadsheet: Oysterlarvaeexposure3122008indivdata, Worksheet: Week[x] buckets [date] 2016-06-28 publication Spreadsheet: Oysterlarvaeexposure3122008indivdata, Worksheet: Wk3 final 3-28-2008 2016-06-28 publication Spreadsheet: Oysterlarvaeexposure3122008indivdata, Worksheet: Wk3 stats 3-28-2008 2016-06-28 publication dataset Accuracy Larval counts are performed on a random 1ml subsample raken from a well-mixed, 250ml concentrated volume of larvae in seawater. Completeness Report On rare occasion, a count may be missing from the database due to accidental loss of sample. Fisher's Island oysters spawned at the Milford Laboratory, and larval bioassays run with 4 treatments: no bacteria control, probiotic OY15, pathogen B183 (Vibrio Corallilyticus) and combination treatment with OY15+B183. Bacteria were dosed every other day and larvae subsamples were taken for survival counts.