gov.noaa.nmfs.inport:35937 eng UTF8 dataset MS Excel Alaska Fisheries Science Center 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 0700-1700 Pacific Time resourceProvider 2026-03-04T19:17:17 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) AFSC/RACE/SAP/Shavey: DNA extraction from archived Giemsa-stained blood smears using polymerase chain reaction to detect host and parasitic DNA AFSC/RACE/SAP/Shavey: DNA extraction from archived Giemsa-stained blood smears using polymerase chain reaction to detect host and parasitic DNA publication NOAA/NMFS/EDM 35937 https://www.fisheries.noaa.gov/inport/item/35937 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Alaska Fisheries Science Center (206) 526-4000 (206) 526-4004 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA afsc.webmaster@noaa.gov https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center WWW:LINK-1.0-http--link Alaska Fisheries Science Center Website AKFSC Home Page information 0700-1700 Pacific Time originator tableDigital These are the data from a laboratory experiment in which DNA content was removed from blood smears and extracted. The blood smears were either stained with Giemsa-stain or remained un-stained. Some of the un-stained smears were coverslipped. Host and parasitic DNA was amplified using conventional PCR. To identify a new technique of removing contents from blood smears and to evalutate the effects of Giemsa-staining and coverslipping for DNA amplication. completed Lang, Christie christie.lang@noaa.gov pointOfContact Lang, Christie christie.lang@noaa.gov custodian notPlanned Blood Smear DNA Extraction disease theme Alaska Bering Sea Gulf of Alaska Newfoundland, Canada SE Alaska Washington State Coast place DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Shellfish Assessment Program project InPort otherRestrictions Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/SAP/Shavey: DNA extraction from archived Giemsa-stained blood smears using polymerase chain reaction to detect host and parasitic DNA [Data Date Range], https://www.fisheries.noaa.gov/inport/item/35937. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. otherRestrictions Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgementof NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. otherRestrictions Distribution Liability: The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 35937 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/35937.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US biota oceans Excel -178.51 -53.11 49.63 61.67 Eastern Bering Sea, Alaska; Gulf of Alaska; Southeast Alaska, Washington State Coast, North Atlantic Coast | Currentness: Ground Condition 1988 2004 false eng false Spreadsheet Blood smear data 2016-12-16 publication Foy, Robert robert.foy@noaa.gov distributor https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Shavey%3B%20DNA%20extraction%20from%20archived%20Giemsa-stained%20blood%20smears%20using%20polymerase%20chain%20reaction%20to%20detect%20host%20and%20parasitic%20DNA.csv WWW:LINK-1.0-http--link https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Shavey%3B%20DNA%20extraction%20from%20archived%20Giemsa-stained%20blood%20smears%20using%20polymerase%20chain%20reaction%20to%20detect%20host%20and%20parasitic%20DNA.csv Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov download dataset Accuracy Data is submitted through a thorough QA/QC process. Completeness Report Due to small sample size, only blood smears with high parasite ratings were used in statistical analysis. Crab morphometrics are not available for some samples. BCS smears were rated with trophont stage. Two samples used in analysis had a trophont and prespore stage (thought to be advanced stage) and those samples were grouped as highest trophont stage (T5). Conceptual Consistency Data is checked for values outside range. A subset of 76 blood smears that were prepared in the field from 1988 to 2004 were chosen based on smear preparation (Giemsa-stained vs. no stain and coverslipped vs no coverslip) and parasite status (parasite presence vs. no parasite). All content was removed from each smear using a pipette tip and sterile blade. DNA was extracted and amplified using crab host and parasite PCR primer sets. SPNO is a number assigned to each collected crab. Duplicate numbers represent replicate smears taken from sample. The sex of the crab is identified as: 1) Male, 2) Female, 3) Unknown. Individual crab carapaces were measured (1 mm), excluding spines, and are reported as size. Null or blank fields in data represent no size information reported for crab specimen. Shell condition class serves as a semi-quantitative index of molt status and time in shell post-molt. Carapace shell condition was assessed for each Chionoecetes crab sampled and assigned to one of six classes according to specific criteria (0 = premolt or molting, 1 = soft and pliable, 2 = new hardshell both firm and clean, 3 = oldshell slightly worn, 4 = oldshell worn, 9 = no shell condition information reported for crab specimen). Collection location was not a factor, so only general location is listed. Blood smears analyzed were either unstained (none), or fixed with methanol and Giemsa-stained for 30 minutes (Giemsa). Treatment of blood smears are identified as (0 = no cover slip, 1 = cover slip attached using mounting media). 30 fields at 250X were read to determine presence or absence of Hematodinium spp. parasite on blood smear. Results were documented as: (0 = Hematodinium parasite absent on smear, 1 = Hematodinium parasite present on smear). Hematodinium parasite presence on smear was identified as T-rating (0 = No rating due to parasite absence, T1 = Up to 10% Hematodinium parasite present on smear (trophont stage), T2 = 10 - 40% Hematodinium parasite present on smear (trophont stage), T3 = 40 - 60% Hematodinium parasite present on smear (trophont stage), T4 = 60 - 90% Hematodinium parasite present on smear (trophont stage), T4P1 = 60 - 90% Hematodinium parasite present on smear (trophont stage) and up to 10% Hematodium prespore stage, T5 = Above 90% Hematodinium parasite present on smear (trophont stage), T5P1 = Above 90% Hematodinium parasite present on smear (trophont stage) and up to 10% Hematodium prespore stage). PCR assays: for 16S, COI, 18S and ITS Primer (0 = Did not use primer set on this sample, 1 = Used primer set on this sample). For 16S, COI, 18S and ITS Amplification (0 = No DNA amplification using this primer set, 1 = DNA was amplified using primer set, 9 = no amplification to report due to primer set not used in sample). Primer set descriptions: 16S = 16Sar-L (CgC CTg TTT ATC AAA AAC AT) & 16Sbr-H (CCg gTC TgA ACT CAg ATC ACg T) primer set used in PCR assay, targeting mitochondrial DNA 16S ribosomal RNA gene. COI = LCO1490 (ggT CAA CAA ATC ATA AAg ATA TTg g) & HCO2198 (TAA ACT TCA ggg TgA CCA AAA AAT CA) primer set used in PCR assay, targeting the mitochondrial cytochrome c oxidase subunit I gene. 18S = HF1487 (CCT ggC TCg ATA gAg TTg) & HR1654 (ggC TgC CgT CCg AAT TAT TCA C) primer set used in PCR assay, targeting the 18S ribosomal gene located at the small subunit 3'-end of the ribosomal RNA unit. ITS = HSP7f (AGT CAT CAG CTC GTG CTG A) & HSP9r (TTC ACG GAA TTC TGC AAT TCG) primer set used in PCR assay, targeting the first internal transcribed spacer of the ribosomal RNA unit. Statistical analysis: Blood smears were analyzed using two-sample t-test (0 = smear was not used in statistical analysis, 1 = smear was used in statistical analysis).