gov.noaa.nmfs.inport:33529 eng UTF8 dataset Alaska Fisheries Science Center 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 0700-1700 Pacific Time resourceProvider 2025-05-15T19:15:53 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) AFSC/RACE/SAP/Swiney: Effects of ocean acidification and increased temperatures on juvenile red king crab AFSC/RACE/SAP/Swiney: Effects of ocean acidification and increased temperatures on juvenile red king crab publication NOAA/NMFS/EDM 33529 https://www.fisheries.noaa.gov/inport/item/33529 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Swiney, Katherine katherine.swiney@noaa.gov originator Long, Chris chris.long@noaa.gov email originator Foy, Robert robert.foy@noaa.gov originator tableDigital Multiple stressor studies are needed to better understand the effects of oceanic changes on marine organisms. To determine the effects of near-future ocean acidification and warming temperature on juvenile red king crab (Paralithodes camtschaticus) survival, growth, and morphology, we conducted a long-term (184 d) fully crossed experiment with two pHs and three temperatures: ambient pH (~7.99), pH 7.8, ambient temperature, ambient +2 degree C, and ambient +4 degree C, for a total of 6 treatments. The purpose of this study was to understand the effects of ocean acidification and warming temperatures on juvenile red king crab survival, growth and morphology. completed Swiney, Katherine katherine.swiney@noaa.gov pointOfContact Swiney, Katherine katherine.swiney@noaa.gov email custodian notPlanned juvenile morphology ocean acidification ocean warming survival young-of-the-year theme DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Shellfish Assessment Program project InPort otherRestrictions Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/SAP/Swiney: Effects of ocean acidification and increased temperatures on juvenile red king crab [Data Date Range], https://www.fisheries.noaa.gov/inport/item/33529. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: Contact Point Of Contact for data request form. otherRestrictions Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. otherRestrictions Distribution Liability: The user is responsible for the results of any application of this data for other than its intended purpose. NOAA denies liability if the data are misused. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 33529 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/33529.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US oceans Excel 170 -130 50 75 Alaskan waters | Currentness: Ground Condition 2012-08-01 2013-02-01 false eng false carapace measurements 2016-07-28 publication growth 2016-07-28 publication intermolt duration 2016-07-28 publication daily pH temp 2016-07-28 publication water chemistry 2016-07-28 publication survival 2016-07-28 publication Swiney, Katherine katherine.swiney@noaa.gov distributor https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Swiney%3B%20Effects%20of%20ocean%20acidification%20and%20increased%20temperatures%20on%20juvenile%20red%20king%20crab WWW:LINK-1.0-http--link https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Swiney%3B%20Effects%20of%20ocean%20acidification%20and%20increased%20temperatures%20on%20juvenile%20red%20king%20crab Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/16/2021. Contact: Daniel.woodrich@noaa.gov download dataset Completeness Report Some of the carapaces were broken or damaged so all measurements could not be taken on them. Conceptual Consistency Data was checked for outliers which were removed. Seawater was acidified using the same methods described in Long et al. (2013a). Briefly, sand filtered seawater was pumped into the Alaska Fisheries Science Center’s Kodiak Laboratory seawater facility from 15 and 26 m depth intakes in Trident Basin. A tank of pH 5.5 was established by bubbling CO2 into ambient seawater. This pH 5.5 water was mixed with ambient seawater in the treatment head tanks via peristaltic pumps controlled by Honeywell controllers and Durafet III pH probes. The ambient head tank did not receive any pH 5.5 water. Waters from the treatment head tanks were then supplied to the treatment tubs. To heat the water, a 200W submersible heater was placed in each heated treatment tub. In the coldest months of the experiment, a 100W heater was added to the warmest treatments to maintain the correct temperatures. Temperature and pHF (free scale) were measured daily from three random inserts in each treatment tub (see below for experimental set-up details) using a Durafet III pH probe calibrated with a TRIS buffer, and when the pH deviated from the target pH by more than ±0.02 pH units, the Honeywell controller set points were adjusted to bring the pH back to the target value. Heater set points were changed manually based upon ambient temperature for each day to maintain target treatment temperatures. Weekly water samples from the treatment head tanks were taken, fixed with mercuric chloride, and sent to an analytic laboratory for dissolved inorganic carbon (DIC) and total alkalinity (TA) analysis. Two laboratories which used similar, but slightly different, instruments were used during the course of the study. At the first laboratory, DIC was determined using a CM5014 Coulometer with a CM5130 Acidification Module (UIC Inc., Joliet, IL) using Certified Reference Material from the Dickson Laboratory (Scripps Institute, San Diego, CA) (Dickson et al., 2007). The TA was measured via open cell titration according to the procedure in Dickson et al. (2007). At the second laboratory, DIC and TA were determined using a VINDTA 3C (Marianda, Kiel, Germany) coupled to a 5012 Coulometer (UIC Inc., Joliet, IL) using Certified Reference Material from the Dickson Laboratory (Scripps Institute, San Diego, CA) and the procedures in DOE (1994). Laboratory Study Ethical approval for this research was not required by any federal, state, or international laws because the study was conducted on invertebrates which are not covered under these laws. Young-of-the-year were reared in the Kodiak Laboratory from an ovigerous female collected in Bristol Bay, Alaska, June 2011 and shipped live to the laboratory. Thirty young-of-the-year crab were randomly assigned to each of 6 treatments: 1) ambient pH, ambient temperature, 2) ambient pH, ambient +2ºC, 3) ambient pH, ambient +4ºC, 4) pH 7.8, ambient temperature, 5) pH 7.8, ambient +2ºC, and 6) pH 7.8, ambient +4ºC. Each treatment was contained in a 53 (L) x 38 (W) x 23 (H) cm tub. Each tub had a flow rate of 600 mL min-1 from the head tank. The young-of-the-year were reared in individual inserts constructed from 40 mm inner diameter PVC pipe with 750 µm mesh attached to the bottom and the inserts were placed inside the treatment tub. The area of this insert was determined to be optimal for individual rearing of juvenile red king crab of the size used in this study (Swiney et al., 2013b). Inserts were placed on plastic grating to raise them off the bottom of the tub. Water was delivered into each insert from above via a submersible pump connected to a manifold which recirculated water within each tub. A temperature logger that recorded data every 30 minutes was placed into each tub. To acclimate the crab to the treatment temperatures, each day the temperature in the tubs was increased by 1ºC until the desired temperature was obtained. Day 1 of the experiment (August 5, 2012) was marked when all of the treatments reached the correct temperature; the experiment ran for 184 d. Throughout the duration of the experiment, crab were fed a gel diet of Gelly Belly (Florida Aqua Farms, Inc., Dade City, FL, USA) enhanced with Cyclop-eeze powder (Argent Laboratories, Redmond, WA, USA), pollock bone powder (US Department of Agriculture, Agricultural Research Service, Kodiak, AK, USA), and astaxanthin (Daly et al., 2012). Crab were fed to excess 3 times per week and old food was removed prior to each feeding. Each insert was checked daily for exuvia and mortalities which were removed for growth and morphometric analysis. Carapaces were carefully removed from all exuvia and mortalities and photographed under a dissecting microscope. Carapace width, carapace length, rostrum base width, orbital spine width, and the first spine length (Figure 1) were measured in mm using Image Pro Plus v. 6.00.260 imaging software (Media Cybernetics, Inc., Bethesda, MD, USA, Long et al., 2013b).