gov.noaa.nmfs.inport:28298 eng UTF8 dataset CSV Files Alaska Fisheries Science Center 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 0700-1700 Pacific Time resourceProvider Bahl, Kimberly 206 526 4314 kimberly.bahl@noaa.gov pointOfContact 2024-02-29T00:00:00 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) AFSC/RACE/EcoFOCI - Walleye Pollock larvae condition data measured in support of EcoFOCI assessment surveys and ecosystem observations in the southeastern Bering Sea and western Gulf of Alaska from 2007 to 2014 AFSC/RACE/EcoFOCI - Walleye Pollock larvae condition data in the southeastern Bering Sea and western Gulf of Alaska 2007 - 2014 publication NOAA/NMFS/EDM 28298 https://www.fisheries.noaa.gov/inport/item/28298 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Porter, Steve steve.porter@noaa.gov originator documentDigital The datasets contain larval size, water temperature, cell cycle data, and classification of larval condition. The datasets were developed to assess the condition of Walleye Pollock larvae using larval size, water temperature, and cell cycle data measured with flow cytometry. underDevelopment Porter, Steve steve.porter@noaa.gov pointOfContact Porter, Steve steve.porter@noaa.gov custodian irregular condition fish larvae theme Bering Sea Gulf of Alaska place DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Recruitment Processes Program project InPort otherRestrictions Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/EcoFOCI - Walleye Pollock larvae condition data measured in support of EcoFOCI assessment surveys and ecosystem observations in the southeastern Bering Sea and western Gulf of Alaska from 2007 to 2014 [Data Date Range], https://www.fisheries.noaa.gov/inport/item/28298. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: Contact point of contact. otherRestrictions Use Constraints: Contact point of contact. unclassified N/A N/A NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 28298 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/28298.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US oceans -180 -150 50 65 | Currentness: Ground Condition 2007 2014 Supplemental Information - Data Parameters and Units - Descriptive Information about the Data Parameters and Units. The datasets contain larval standard length measured in mm, the proportion of cells in the S and G2/M phases of the cell cycle, and the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content measured with flow cytometry. Also included is the mean water temperature (°C) between the surface and 40 m depth. Supplemental Information – Methods - Descriptive Information about the methods used. Larvae were collected using a 60 cm bongo frame fitted with 0.505 mm mesh nets and were frozen at -80°C. Tissue preparation, flow cytometry protocol, and data analysis are described in Porter and Bailey (2011 and 2013). Supplemental Information – Instruments - Descriptive Information about the instruments and equipment used. A BD Biosciences Influx flow cytometer (BD Biosciences, San Jose, CA) was used to analyze each larva. The proportion of nuclei in the S, and G2/M phases was calculated for each larva with MultiCycle AV software, vers. 4.0 (Phoenix Flow Systems, San Diego, CA). FCS Express flow cytometry analysis software, vers. 3.0 (De Novo Software, Los Angeles, CA) was used to calculate the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content. A detailed description of the analyses is given in Porter and Bailey 2013. Supplemental Information - Sampling Scales and Rates - Descriptive Information about the sampling spatial and temporal scales and rates. Samples were taken from the southeastern Bering Sea and western Gulf of Alaska, and were conducted opportunistically over multiple years. Supplemental Information - Error Analysis - Descriptive Information about the error analysis. Supplemental Information - Provenance and Historical References - Descriptive Information about the provenance, historical data, key information packaged elsewhere. PORTER, S.M., and K.M. BAILEY. 2011. Assessing the condition of walleye pollock Theragra chalcogramma (Pallas) larvae using muscle-based flow cytometric cell cycle analysis. J. Exp. Mar. Biol. Ecol. 399:101-109. PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear RNA with flow cytometry to improve assessment of larval condition of walleye pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351. CSV - Comma Separated Values (Text) Porter, Steve steve.porter@noaa.gov distributor https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/2007%20walleye%20pollock%20larvae%20condition.csv WWW:LINK-1.0-http--link https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/2007%20walleye%20pollock%20larvae%20condition.csv Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov download dataset Representativeness N/A Accuracy N/A Analytical Accuracy N/A Quantitation Limits N/A Bias N/A Comparability N/A Completeness Measure N/A Precision N/a Analytical Precision N/A Field Precision N/A Sensitivity N/A Detection Limit N/A Completeness Report N/A Conceptual Consistency N/A A BD Biosciences Influx flow cytometer (BD Biosciences, San Jose, CA) was used to analyze each larva. The proportion of nuclei in the S, and G2/M phases was calculated for each larva with MultiCycle AV software, vers. 4.0 (Phoenix Flow Systems, San Diego, CA). FCS Express flow cytometry analysis software, vers. 3.0 (De Novo Software, Los Angeles, CA) was used to calculate the ratio of the number S phase nuclei to the number of G1 phase nuclei with high nRNA content. A detailed description of the analyses is given in Porter and Bailey 2013. otherRestrictions otherRestrictions Access Constraints: None | Use Constraints: None