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        <gco:CharacterString>To study the effects of ocean acidification we examined the effects of ocean acidification on the larval stages of the economically important southern Tanner crab, Chionoecetes bairdi. 
                Ovigerous females were reared in one of 3 treatments: control (ambient pH ~8.1), pH 7.8, and pH 7.5 for 2 years. Larvae in year 1 were from oocytes developed in the field whereas larvae in year 
                2 were from oocytes developed under acidified conditions. Larvae hatched each year, were also exposed to 3 pH treatments to examine starvation-survival, morphology, condition, and calcium/magnesium 
                content. Approximately 300 larvae were stocked in multiple treatments for testing the effect of pH. Hatching success was measured as the total % of larval hatched from a full clutch while duration 
                was the number of days over which hatching occurred. Hatching success did not differ among treatments in 2012 but varied between 46 to 87% in 2013 dependent on pH treatment. Larval mass was highest 
                in pH 7.8 in 2012 and lowest in the control, however in 2013 the highest larval mass was in the control water. There were only small (not significant) changes in magnesium or calcium content among 
                treatments in 2012 however, the reduction in both minerals at higher pH was greater in 2013. There was higher percent carbon and nitrogen contents in pH 7.5 larvae in 2013. The morphology of Tanner 
                crab larval was assessed from 200 larvae stocked in multiple 2 L beakers. There was no effect of treatment on larval morphometrics. In 2012 and 2013, we examined if embryos developed under acidified 
                conditions affected larval morphology by assessing 15 newly hatched larvae from each treatment. There was again no effect of treatment on larval morphometrics. Starvation survival experiments 
                were performed in 1 L sized PVC inserts. In both years larvae from embryos that developed in pH 7.5 water survived about 3 days longer than those that developed in control water. However, in 2012 
                larvae from embryos that had developed in pH 7.8 water were similar to control larvae whereas in 2013 they were intermediate between the control and pH 7.5 larvae.  The overall effects of treatment 
                at the larval stage appeared to be better condition and initial survival at lower pH, however multiple years of treatment led to lower survival.</gco:CharacterString>
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          </gmd:evaluationMethodDescription>
          <gmd:result gco:nilReason="missing" />
        </gmd:DQ_CompletenessCommission>
      </gmd:report>
      <gmd:report>
        <gmd:DQ_ConceptualConsistency>
          <gmd:nameOfMeasure>
            <gco:CharacterString>Conceptual Consistency</gco:CharacterString>
          </gmd:nameOfMeasure>
          <gmd:evaluationMethodDescription>
            <gco:CharacterString>Hatching success and duration, larval morphology, and Ca and Mg information were broadly compared to previous studies on Tanner crab mineral content and
            to similar measurements made on similar crab species and statistically compared to a control (alpha=0.05). Larval mass and survival information was compared 
            broadly to known values and statistically compared to a control (alpha=0.05); replicate response was also considered. 
            For example, for comparing we used the study described in Mathis, Jeremy T., Jessica N. Cross, 
            and Nicholas R. Bates. "Coupling primary production and terrestrial runoff to ocean acidification and carbonate 
            mineral suppression in the eastern Bering Sea." Journal of Geophysical Research: Oceans (19782012) 116.C2 (2011).</gco:CharacterString>
          </gmd:evaluationMethodDescription>
          <gmd:result gco:nilReason="missing" />
        </gmd:DQ_ConceptualConsistency>
      </gmd:report>
      <gmd:lineage>
        <gmd:LI_Lineage>
          <gmd:statement gco:nilReason="missing" />
          <gmd:processStep>
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Measuring of Hatching success, larval mass, Ca and Mg, CHN, and survival data</gco:CharacterString>
              </gmd:description>
              <gmd:dateTime>
                <gco:DateTime>2013-07-30T00:00:00</gco:DateTime>
              </gmd:dateTime>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Analysis of the results</gco:CharacterString>
              </gmd:description>
              <gmd:dateTime>
                <gco:DateTime>2014-05-07T00:00:00</gco:DateTime>
              </gmd:dateTime>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Collection of larval Morphology data 1 step</gco:CharacterString>
              </gmd:description>
              <gmd:dateTime>
                <gco:DateTime>2011-07-21T00:00:00</gco:DateTime>
              </gmd:dateTime>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Collection of larval Morphology data 2 step</gco:CharacterString>
              </gmd:description>
              <gmd:dateTime>
                <gco:DateTime>2013-05-29T00:00:00</gco:DateTime>
              </gmd:dateTime>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Statistical analysis</gco:CharacterString>
              </gmd:description>
              <gmd:dateTime>
                <gco:DateTime>2014-04-07T00:00:00</gco:DateTime>
              </gmd:dateTime>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
</gmi:MI_Metadata>