gov.noaa.nmfs.inport:26894
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dataset
Alaska Fisheries Science Center
7600 Sand Point Way N.E., Building 4
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WA
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USA
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2025-05-15T19:15:47
ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data
ISO 19115-2:2009(E)
AFSC/RACE/SAP/Long: Data from: Embryo development in golden king crab, Lithodes aequispina.
AFSC/RACE/SAP/Long: Data from: Embryo development in golden king crab, Lithodes aequispina.
publication
NOAA/NMFS/EDM
26894
https://www.fisheries.noaa.gov/inport/item/26894
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View the complete metadata record on InPort for more information about this dataset.
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tableDigital
The data from this study, describes embryo development in Golden king crab, Lithodes aequispinus. Six female multiparous golden king crab were captured from the Aleutian Islands, Alaska, and mated in the laboratory. Their embryos were photographed and staged on average once every 9 days throughout embryogenesis.
The purpose of this data is to describe embryogenesis in golden king crab.
We thank B. Stevens for training, sharing techniques, and helpful suggestions, K. Reppond for assistance, and the staff, interns, and volunteers of the Kodiak Seawater Laboratory for assistance in collecting the data.
underDevelopment
Long, Chris
chris.long@noaa.gov
email
pointOfContact
Long, Chris
chris.long@noaa.gov
email
custodian
asNeeded
eggs
reproduction
theme
Aleutian Islands
Bering Sea
place
DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2017-04-24
publication
8.5
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
GCMD Keyword Forum Page
Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
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Shellfish Assessment Program
project
InPort
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Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/SAP/Long: Data from: Embryo development in golden king crab, Lithodes aequispina. [Data Date Range], https://www.fisheries.noaa.gov/inport/item/26894.
NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
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Access Constraints: The data set is still being analyzed and will not be available for distribution until it has been finalized and all QA/QC practices have been performed. Contact the Point Of Contact for estimated time of release.
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Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
unclassified
NOAA Data Management Plan (DMP)
NOAA/NMFS/EDM
26894
https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/26894.pdf
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NOAA Data Management Plan (DMP)
NOAA Data Management Plan for this record on InPort.
information
crossReference
eng; US
oceans
Excel
-152.395268
-152.385268
57.782403
57.792403
Kodiak Fisheries Research Center
| Currentness: Ground Condition
2006-09-01
2008-08-01
false
eng
false
Spreadsheet
Crab data
2019-02-22
publication
Embryo Stages
2019-02-22
publication
Embryo measurements
2019-02-21
publication
Long, Chris
chris.long@noaa.gov
email
distributor
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Embryo%20development%20in%20golden%20king%20crab%2C%20Lithodes%20aequispina%3B%20Crab%20Data.csv
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https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Embryo%20development%20in%20golden%20king%20crab%2C%20Lithodes%20aequispina%3B%20Crab%20Data.csv
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021. Contact: Daniel.woodrich@noaa.gov
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Accuracy
Data was checked for outliers and for values out of expected range.
<methtype>Lab</methtype>
<methdesc>Golden king crab for this experiment were caught in commercial pots along the Aleutian Islands, Alaska, in the falls of 2005 and 2006 and transported to the ASFC Kodiak Laboratory via air cargo. Crab were held in 2,000 l tanks with flow-through seawater chilled to 3-4 degrees Celsius, and fed chopped frozen fish and squid to excess. Tanks were covered with opaque sheets of foam to both provide insulation and to keep the females in mostly-dark conditions. Six females, 4 captured in 2005 and 2 in 2006, were used in this study. Females were either late-stage ovigerous, as evinced by eyed embryos, or hatched out, as evinced by empty egg cases when collected and were between 120 and 141 mm in carapace length (CL). Six mature males, CL 128-132 mm, were present with the females in a pre-mating holding tank. After the females molted, they mated and extruded a new clutch of eggs. At this point they were placed into another tank where all the post-mating females were held together with no males.</methdesc>
</method>
<method>
<methtype>Lab</methtype>
<methdesc>Embryos were collected regularly throughout development from a random location within the clutch. Time between samples varied with the developmental stage and averaged once every 9 days or a mean of 48 times (range 42-54) throughout development for each female. At sampling, embryos were examined and photographed under a stereo microscope. Uneyed embryos were stained for 5 min in Bouin's solution to enable staging. The median stage of development (see results for descriptions) was determined for each female on each sampling day. In addition, up to 10 unstained embryos were photographed at 90° to the sagittal plane under a stereo microscope for image analysis. The images were calibrated each day with a micrometer because the scope had an adjustable zoom and this ensured accurate measurements. Measurements were made using Image-Pro Plus V. 7.0 (Media Cybernetics, Inc. , Rockville, MD). In this data, the term egg; refers to the entire embryo (i.e. the entire contents of the fertilized egg) and the term embryo; refers to the differentiated part of the egg as distinct from the yolk, and yolk; refers to the undifferentiated deutoplasm. For each egg, the area and the mean, minimum, and maximum diameter of the egg was measured. The mean, minimum, and maximum diameters were determined from 180 measurements of the diameter at 2 degree intervals around the entire egg. When the embryo became visible, the yolk area was also measured by tracing the yolk in Image-Pro and the percent area yolk (yolk area x 100/egg area; prior to the embryo becoming the yolk area = egg area) and the embryo area (egg area minus the yolk area) were calculated. Finally, when the eyes became visible, the eye area and the mean, minimum, and maximum diameter of the eyes were measured using the same techniques as above.</methdesc>
</method>