gov.noaa.nmfs.inport:21948 eng UTF8 dataset Database Alaska Fisheries Science Center 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 0700-1700 Pacific Time resourceProvider 2026-03-04T19:17:17 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) AFSC/RACE/EcoFOCI: NPRB_1220:Mitochondrial DNA-based identification of eggs, larvae and dietary components of commercially and ecologically important fish species and selected invertebrates in the northeast Pacif AFSC/RACE/EcoFOCI: NPRB 1220:Mitochondrial DNA-based identification of eggs, larvae and dietary components of commercially and ecologically im publication NOAA/NMFS/EDM 21948 https://www.fisheries.noaa.gov/inport/item/21948 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information Canino, Michael F 206 526 4108 mike.canino@noaa.gov originator Alaska Fisheries Science Center (206) 526-4000 (206) 526-4004 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA afsc.webmaster@noaa.gov https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center WWW:LINK-1.0-http--link Alaska Fisheries Science Center Website AKFSC Home Page information 0700-1700 Pacific Time originator tableDigital Accurate identification of various life history stages and prey items of marine fishes and invertebrates is central for understanding distribution,abundance, trophic ecology, and biodiversity of these species. Taxonomic approaches have been successfully applied to ichthyoplankton identification and diet analysis efforts for many years. Identification to the species level requires varying degrees of taxonomic expertise. and diagnostic characters for eggs or larvae in some species have not been elucidated. In the current dataset we assembled a mitochondrial DNA (mtDNA) database for which used standard laboratory protocols (restriction fragment length polymorphism and Sanger DNA sequencing) to accurately identify any life history stages of selected fish and shrimp species, with special emphasis on those species that have been difficult or impossible to identify by conventional taxonomic means. Fish and shrimp specimens were collected between 2010 - 2013. We developed a restriction fragment length polymorphism (RFLP)protocol, based upon mitochondrial DNA sequences to distinguish between Pacific halibut and Greenland halibut, but were unable to develop one to discriminate between Bering flounder and flathead sole. We used direct Sanger sequencing of mitochondrial DNA for species identification of 32 species of sculpin and four species of caridean shrimp. PCR products from fish and shrimp samples were sequenced using an ABI 3730 automated sequencer (Applied Biosystems, Inc). DNA sequences from museum voucher specimens were compared with entries in the public databases for those species. Sequences from voucher specimens have greater taxonomic authority for use in species identification than those without vouchers, adding greater confidence to species identifications based upon our data. To develop cost effective molecular protocols for accurate identification of flatfish eggs, larvae and digested prey remains of sculpins and caridean marine shrimps. completed Canino, Michael F 206 526 4108 mike.canino@noaa.gov pointOfContact Canino, Michael F 206 526 4108 mike.canino@noaa.gov email custodian notPlanned Non-Federal Funding theme PARR Exclusion DNA barcoding ND1 control region cytochrome b cytochrome oxidase gut contents mitochondrial DNA species identification theme Alaska Bering Sea Chuckchi Sea Oregon State Puget Sound Washington State place DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Recruitment Processes Program project InPort otherRestrictions Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/EcoFOCI: NPRB_1220:Mitochondrial DNA-based identification of eggs, larvae and dietary components of commercially and ecologically important fish species and selected invertebrates in the northeast Pacif [Data Date Range], https://www.fisheries.noaa.gov/inport/item/21948. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. otherRestrictions Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgementof NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 21948 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/21948.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US biota FAS files can bge worked in any simple text editor such as notepad, MS Word 2012 -178.46 -120.1 42.42 71.5 Chukchi Sea, Bering Sea, Gulf of Alaska, northeastern Pacific Ocean, Washington State, Puget Sound, Oregon State | Currentness: Ground Condition 2000 2013 Canino, Michael F 206 526 4108 mike.canino@noaa.gov distributor https://ncei.noaa.gov WWW:LINK-1.0-http--link https://ncei.noaa.gov download dataset Accuracy Molecular size standards and restriction maps Completeness Report Base calls with PHRED scores less than 19 were omitted from analysis. Novel mutations were verified by sequencing DNA segments in both directions. unknown otherRestrictions otherRestrictions Access Constraints: none | Use Constraints: none