gov.noaa.nmfs.inport:12673 eng UTF8 dataset CSV Files Alaska Fisheries Science Center 7600 Sand Point Way N.E., Building 4 Seattle WA 98115 USA https://www.afsc.noaa.gov WWW:LINK-1.0-http--link Website Website for this organization information 0700-1700 Pacific Time resourceProvider Bahl, Kimberly 206 526 4314 kimberly.bahl@noaa.gov email pointOfContact 2024-02-29T00:00:00 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for imagery and gridded data ISO 19115-2:2009(E) AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis AFSC/RACE/EcoFOCI: NPRB project 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flo publication NOAA/NMFS/EDM 12673 https://www.fisheries.noaa.gov/inport/item/12673 WWW:LINK-1.0-http--link Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information tableDigital Walleye pollock are an important component of the eastern Bering Sea ecosystem due to their vast numbers and biomass and are of great commercial importance. Their recruitment in the eastern Bering Sea is not well understood but it is largely determined during the first year, so the larval stage may play a significant role. Starvation may contribute to the high and variable mortality that occurs during the larval stage so it is important to monitor and quantify it. This metadata describes data from laboratory and field studies conducted from 2009 to 2012 for NPRB project 926. The project completed the development of an assay using flow cytometric cell cycle analysis to assess the physiological condition of walleye pollock larvae, and applied it to field collected specimens. The use of RNA to improve the accuracy of the assay was also investigated, as well as the relationship between flow cytometric measurements and growth. Results showed that the overall classification accuracy of the assay (healthy or unhealthy) ranged between 75 and 83% depending on the type of cross-validation testing. A nuclear RNA variable (the ratio of the number of S phase nuclei to the number of high nuclear RNA content G1 phase nuclei) improved overall classification accuracy by 11% compared to a model without it. Unhealthy walleye pollock larvae were present in the southeastern Bering Sea in 2009, and this was most likely the result of cold environmental conditions affecting prey availability and larval feeding. In 2010, no unhealthy larvae were detected. This may be the result of less spatial coverage and smaller collections of larvae than in 2009, given that the environmental conditions were similar between years. To complete the development of an assay to assess the physiological condition of walleye pollock larvae using flow cytometric cell cycle analysis, and to apply it to field collected . completed Bahl, Kimberly 206 526 4314 kimberly.bahl@noaa.gov email pointOfContact Porter, Steve steve.porter@noaa.gov email custodian notPlanned Non-Federal Funding theme PARR Exclusion flow cytometry larvae physiological condition theme Alaska Bering Sea place DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2017-04-24 publication 8.5 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Recruitment Processes Program project InPort otherRestrictions Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis [Data Date Range], https://www.fisheries.noaa.gov/inport/item/12673. NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose. otherRestrictions Access Constraints: Contact Point Of Contact for data request form. otherRestrictions Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested. unclassified NOAA Data Management Plan (DMP) NOAA/NMFS/EDM 12673 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/12673.pdf WWW:LINK-1.0-http--link NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information crossReference eng; US biota Excel -171.1 -163.3 54.3 57.4 Bering Sea | Currentness: Ground Condition 2009 2012 CSV - Comma Separated Values (Text) Porter, Steve steve.porter@noaa.gov email distributor https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv WWW:LINK-1.0-http--link https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/14/2021. Contact: Daniel.woodrich@noaa.gov download dataset Completeness Report Not applicable. PORTER, S. M., and K. M. BAILEY. 2013. Using measurements of muscle cell nuclear RNA with flow cytometry to improve assessment of larval condition of walleye pollock (Gadus chalcogrammus). Fish. Bull., U.S. 111:337-351. otherRestrictions otherRestrictions Access Constraints: none | Use Constraints: none