vieques_project_metadata eng; USA utf8 dataset National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment (CCMA) National Status and Trends Program Manager 301-713-3028 1305 East West Highway, N/SCI-1 Silver Spring MD 20910 dave.whitall@noaa.gov pointOfContact 2024-12-15 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) National Status and Trends: Vieques, Puerto Rico Tissue Contaminants Data 2015-11-01 publication Department of Commerce (DOC), National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA) originator NOAA's Ocean Service, National Centers for Coastal Ocean Science (NCCOS) Silver Spring MD publisher PRINCIPAL INVESTIGATORS David Whitall, NOAA Ocean Service-National Centers for Coastal Ocean Science(NCCOS) (NCCOS) SAMPLE COLLECTION INVESTIGATORS David Whitall, NOAA Ocean Service-National Centers for Coastal Ocean Science (NCCOS) Andrew Mason, NOAA Ocean Service-National Centers for Coastal Ocean Science (NCCOS) PROCESSING INVESTIGATORS: Mike Fulton (NOAA Ocean Service-National Centers for Coastal Ocean Science(NCCOS), Ed Wirth (NOAA Ocean Service-National Centers for Coastal Ocean Science (NCCOS) Worldwide coral reef ecosystems are being threatened by a range of human activities. Sedimentation, overfishing, global climate change, ship groundings, pathogens and pollution are some of the major human-related stressors impacting coral reefs. The eastern half of the island of Vieques (Puerto Rico) was used by the U.S. Navy for live fire exercises for over fifty years, ending in 2003. A variety of compounds associated with military activities have the potential to negatively impact coral reef ecosystems. Despite previous studies on soil and biota which found relatively low levels of pollutants in the environment, there is still public concern about the impact of pollution on the fishery. This study assessed tissue concentrations of energetic compounds, DDT and metals in fisheries important species (Queen conch and spiny lobster). The objectives of this study were to: quantify contaminant levels in species important to the Vieques fishery; determine spatial differences within the study area and put findings in a larger context using information available in the scientific literature. This data set was developed by National Oceanographic and Atmospheric Administration (NOAA) completed David Whitall National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal and Ocean Science, Center for Coastal Monitoring and Assessment (CCMA) Principal Investigator/Senior Scientist 301-713-3028 Ext:138 1305 East West Highway, N/SCI-1 Silver Spring MD 20910 dave.whitall@noaa.gov pointOfContact asNeeded CoRIS_Metadata theme NCEI Geoportal Filter Numeric Data Sets > Chemical Contaminants theme CoRIS Discovery Thesaurus EARTH SCIENCE > Human Dimensions > Environmental Impacts > Contaminants theme CoRIS Theme Thesaurus Queen conch chemical contaminants metals DDT energetics organochlorines pesticides coastal monitoring nsandt trace elements land based sources of pollution Unexploded ordnance coastal ecosystem science theme None Environmental Monitoring theme NOS Data Explorer Topic Category L185 Contaminants in Marine Resources of Vieques, PR 20641 Contaminants in the marine resources of Vieques 1085 project CRCP Project United States Puerto Rico place None OCEAN BASIN > Atlantic Ocean > Caribbean Sea /North Atlantic Ocean > Puerto Rico > Puerto Rico (18N066W0000) COUNTRY/TERRITORY > United States of America > Puerto Rico > Puerto Rico (18N066W0000) OCEAN BASIN > Atlantic Ocean > Caribbean Sea /North Atlantic Ocean > Puerto Rico > Vieques Island > Vieques Island (18N065W0002) COUNTRY/TERRITORY > United States of America > Puerto Rico > Vieques > Vieques Island (18N065W0002) place CoRIS Place Thesaurus PR place CoRIS Region These data were prepared by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, make any warranty, expressed or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed in this report, or represents that its use would not infringe privately owned rights. Reference therein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. Any views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Although all data have been used by NOAA, no warranty, expressed or implied, is made by NOAA as to the accuracy of the data and/or related materials. The act of distribution shall not constitute any such warranty, and no responsibility is assumed by NOAA in the use of these data or related materials. otherRestrictions Use Constraints: NOAA requests that all individuals who download NOAA data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: Some or all of the data described in this article were produced by the NOAA's National Ocean Service through its National Status and Trends Program. otherRestrictions Access Constraints: None eng; USA environment oceans -65.5039325 -65.295939167 18.1027579722 18.14773167 2014-08-10 2014-08-16 The units that these parameters are stored in is: in ug/wet g for trace metals and ng/wet g for everything else. The latitude and longitude is measured using datum NAD83. The QA/QC file including Procedural Blanks, Duplicates, Matrix Spikes, Matrix Spike Duplicates and Standard Reference Materials (SRMs) are available upon request. Entity and Attribute Overview: Data Dictionary for the "BAsites" File: SITES http://ccma.nos.noaa.gov/about/coast/nsandt/dictionaries/ba_sites_dictionary.aspx Data Dictionary for all "BA and MW Chemistry" Files: Chem http://ccma.nos.noaa.gov/about/coast/nsandt/dictionaries/ba_mw_chemistry_dictionary.aspx Entity and Attribute Detail Citation: National Status and Trends Program ASCII (tab delimited) ASCII (tab delimited) ASCII (tab delimited) ASCII (tab delimited) ASCII (tab delimited) National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment National Status and Trends Program Manager 301-713-3028 1305 East West Highway, N/SCI-1 Silver Spring MD 20910 dave.whitall@noaa.gov distributor None https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/ information https://cdn.coastalscience.noaa.gov/datasets/nsandt/dataFiles/ss/puertoRico/Sites_2014PuertoRicoSpecialStudy.txt information https://cdn.coastalscience.noaa.gov/datasets/nsandt/dataFiles/ss/puertoRico/2014PuertoRicoSpecialStudy_TraceElements_Tissue.txt information https://cdn.coastalscience.noaa.gov/datasets/nsandt/dataFiles/ss/puertoRico/2014PuertoRicoSpecialStudy_Energetics_Tissue.txt information https://cdn.coastalscience.noaa.gov/datasets/nsandt/dataFiles/ss/puertoRico/2014PuertoRicoSpecialStudy_DDTs.txt information dataset This dataset is complete. All spiking/calibration and internal standard stocks were stored refrigerated in glass amber bottles. Working stocks for spiking and calibration were volumetrically prepared weekly from concentrated stocks in methanol to minimize degradation. Standards for the native compounds were purchased from AccuStandard, while stable isotope-labeled standards were purchased from AccuStandard and Cambridge Isotope Laboratories. Data Acquisition: The conch and lobster samples were taken by NCCOS SCUBA divers. Nitrile gloves were worn to prevent cross contamination between samples. Upon surfacing the samples were placed immediately on ice. At the end of each field day, samples were stored frozen at the USFWS facility in Vieques. At the end of the field mission, the samples were shipped on overnight on blue ice to the analytical lab at NCCOS’ Center for Coastal Environmental Health and Biomolecular Research in Charleston, SC. Sample Collection: Calibration: The sampling gear does not require any calibration, although it was inspected daily for wear and damage.Sample Collection: Quality Control: 2010-03-31T00:00:00 Data Preparation and Sample Processing:Sample Processing Objective: Conch and lobster were received at the laboratory in Charleston, SC and stored at -40C prior to preparation for analysis. Briefly, tissues were prepared after thawing by homogenizing each conch in a Teflon container using a titanium handheld probe homogenizer (ProScientific, Inc.). Only the edible lobster tissue (tail meat) was homogenized for analysis. Homogenized samples were sub-sampled for organic contaminant analysis (energetics, DDTs) and trace metals analysis (Balthis et al., 2012). Moisture content was determined by drying in an oven at 85C (>24 hr) until constant mass was achieved. All data were validated by comparison with blanks, spikes (matrix and reagent spikes), and certified reference materials. Trace metal determination used methods described in Reed et al. (2015). Briefly, homogenized tissue was microwave digested in nitric acid followed by peroxide addition. Analysis for 21 elements (Ag, Al, As, Ba, Be, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, Tl, U, V, and Zn) was achieved using ICP-MS (Perkin Elmer Elan 6100), while Hg was determined by direct mercury analysis (DMA-80, Milestone Inc.). DDTs were determined by GC/MS (Kimbrough et al, 2006) with slight modifications to the protocols. Briefly, samples were prepared for Accelerated Solvent Extraction (ASE) by grinding the sample homogenate with ~28 g anhydrous sodium sulfate in a mortar. Internal standards were added to the samples prior to extraction by ASE. Post extraction clean-up was achieved using gel permeation chromatography, and collected fractions were further processed using activated alumina. Final extracts were analyzed using an Aglient 6890 Gas chromatograph paired with an Agilent 5973 MS. The determination of munition compounds in marine tissues was achieved by two separate extraction methods (Table 2), a modification of EPA 8330B (USEPA 2006) and a Dionex ASE 200 Accelerated Solvent Extraction (ASE). Samples were extracted by both methods. In the modified EPA 8330B method, homogenized sample aliquots (~10 g wet) were lyophilized in amber vials for two days prior for water removal. After lyophilization, samples were transferred to hexane rinsed mortar bowls and ground into a fine powder. Samples were returned to their respective vials and stored in a foil covered desiccator until extraction. Tissues samples of ~1.2 g dry (corresponding to ~4.5 g wet) were placed into 50 ml glass, solvent rinsed centrifuge tubes. The internal standards 13C7, 15N3-TNT, 13C4, 15N4-HMX, 13C3-RDX, d5-Nitrobenzene and 3,4-Dinitrotoluene were added followed by 15 mL of 50:50 dichloromethane/acetone. Centrifuge tubes were capped and vortexed for 1 minute then placed into a chilled sonicator bath for 3 hours with temperature controlled not to exceed 30C. Roughly 4.2 g of wet tissue sample was placed into a solvent rinsed mortar bowl containing ~27 g of anhydrous sodium sulfate. Samples were ground thoroughly and transferred into 33 mL ASE cells. Samples were spiked with the internal standards and extracted using an ASE 200 using a 50:50 Dichloromethane/Acetone mixture at 1000 psi. Calibration standards, reagent spikes, and matrix spikes were extracted by ASE in addition to the samples (range: 10-250 ng). After extraction (sonication or ASE) samples were filtered through sodium sulfate into 200 mL TurboVap tubes and concentrated under a stream of nitrogen (pressure did not exceed 1.1 bar, water bath temperature = 25C). Samples were concentrated to 0.5 mL and solvent exchanged with dichloromethane once. Samples were again concentrated to 0.5 mL and were transferred to glass culture tubes. TurboVap tubes were rinsed three times with dichloromethane and added to the sample extracts (Fv=2 mL). Samples were then cleaned up using a J2 Scientific Gel Permeation Chromatography (GPC) system (J2 Scientific Biobead column with 100% dichloromethane as the mobile phase). After GPC cleanup samples were concentrated to 0.5 mL and solvent exchanged to methanol twice. Extracts were concentrated to 0.5-1 ml and filtered through 0.45um PTFE filters into amber sample vials. Recovery standards were added prior to instrumental analysis (1,2-Dinitrobenzene and d4-17? estradiol) . Sample extracts were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) and gas chromatography mass spectrometry (GC/MS since several analytes would not ionize on the LC-MS/MS platform.) While EPA methods use HPLC-DAD, initial work showed multiple interferences with conch and lobster tissue. For LC-MS/MS, an Agilent 1100 Series HPLC/AB Sciex API 4000 tandem mass spectrometer, operated in negative electrospray ionization with scheduled multiple reaction monitoring, was used. Separation was performed by a Phenomenex Synergi 4u Hydro-RP 80A column using a methanol/water gradient. For GC/MS analysis, an Agilent GC/MS (6890/5973N) operated in selected ion monitoring was used. Samples were injected onto a Restek DB-225ms column (30m x 0.25 um x 0.25 mm) through a split-splitless injector. Calibration curves (10-250 ng) were prepared for each batch of samples (from 8 to 13 samples). For ASE samples, the extracted curve was used, while sonication extracted samples used a calibration curve prepared directly from a stock solution. 2014-10-31T00:00:00 Project related references: Kimbrough, K.L., G.G. Lauenstein, and W.E. Johnson. 2007. Organic Contaminant Analytical Methods of the National Status and Trends Program: Update 2000-2006, NOAA Technical Memorandum, NOS NCCOS 30. 22 Apr. 2008 http://www.ccma.nos.noaa.gov/publications/organicsmethods.pdf. Kimbrough, K.L., and G.G. Lauenstein. 2007. Major and Trace Element Analytical Methods of the National Status and Trends Program: 2000-2006, NOAA Technical Memorandum, NOS NCCOS 29. 22 Apr 2008 http://www.ccma.nos.noaa.gov/publications/nsandtmethods.pdf. McDonald, S. J., D. S. Frank, J. A. Ramirez, B. Wang, and J. M. Brooks. 2006. Ancillary Methods of the National Status and Trends Program: Update 2000-2006, NOAA Technical Memorandum, NOS NCCOS 28. 22Apr. 2008 http://www2.coastalscience.noaa.gov/publications/handler.aspx?resource=3Qy6dD5SKzp6qSIbiw0zZm3IfEn1ajewncp0CWu+1Oc= Apeti, D.A., S.I. Hartwell, W.E. Johnson and G.G. Lauenstein. 2012. National Status and Trends Bioeffects Progam: Field Methods. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Asssessment. NOAA NCCOS Technical Memorandum 135. Silver Spring, MD.27 pp. http://www2.coastalscience.noaa.gov/publications/detail.aspx?resource=kXUVxxA8LH64JetFFum2pA6CRM0NB9yNGhLpzqLyij4= annually This metadata was automatically generated from the Content Standard for Digital Geospatial Metadata standard (version FGDC-STD-001-1998) using the 2016-01-11 version of the FGDC RSE to ISO 19115-2 transform. Translated from FGDC 2025-03-24T19:23:46.726Z Last Metadata Review Date: 2016-12-22 National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment (CCMA) National Status and Trends Program Manager 301-713-3028 1305 East West Highway, N/SCI-1 Silver Spring MD 20910 dave.whitall@noaa.gov pointOfContact