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        <gco:CharacterString>Any differences in sample collection or processing is noted in the methods section below.

Methods for Lake Michigan zooplankton:
Zooplankton were collected with a 0.5 or 1 meter diameter, 153 µm mesh net. The net was vertically towed through the water column at a speed of 0.5 m s-1 from 1 to 2 meters above the bottom to the surface. For partial water column tows, to collect separately the epilimnion, metalimnion and hypolimnion, a messenger actuated choke-off vertical opening/closing net (1m diameter, 2m-long, 153 µm mesh) was used to obtain the zooplankton samples. The net was washed thoroughly, but gently and the contents were carefully transferred to a sample bottle, narcotized with Alka-Seltzer, and preserved with the addition of sugar formaldehyde to form a 2% solution (Haney &amp; Hall, 1973).

Preparation for counting and identifying zooplankton required measurement of the sample volume, gentle mixing of the sample, and removal of an aliquot with a Hensen-Stempel pipette. The aliquot was held in a 100 µm meshed bottom cup and rinsed with tap water to remove the preservative and finally it was poured into a circular counting dish. A minimum 550 zooplankton (or 600 for EEGLE project) were identified for each sample. To count large predatory cladocerans, such as Bythotrephes, the whole sample was rinsed through a 600 µm mesh sieve, and all individuals were counted.

Taxonomic groups were identified and categorized in the following manner. For copepods, both cyclopoid and calanoid nauplii were combined, copepodites were identified to genus and adult copepods were identified to species. The cladocerans, both herbivorous and predatory, were identified to species.

To determine zooplankton biomasses, length measurements were made on a subsample of taxa (10 adult copepods and 25 copepodites or cladocerans) that were over 10% of the total density (or 5% for the EEGLE project) using Image Pro Plus, image analysis software (Media Cybernetics, Silver Spring, MD). In the case of large predatory cladocerans, all individuals were measured or up to 100 individuals if more than that were present. Biomasses were determined using published length-weight regressions (Culver et al. 1985, Makarewicz and Jones 1990). For zooplankton taxa that comprised less than 10% of the total density, a default weight from the literature was used to determine biomass for all taxa except Bythotrephes (Hawkins and Evans 1979).</gco:CharacterString>
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                      <gmd:electronicMailAddress>
                        <gco:CharacterString>ncei.info@noaa.gov</gco:CharacterString>
                      </gmd:electronicMailAddress>
                    </gmd:CI_Address>
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                </gmd:CI_Contact>
              </gmd:contactInfo>
              <gmd:role>
                <gmd:CI_RoleCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact">pointOfContact</gmd:CI_RoleCode>
              </gmd:role>
            </gmd:CI_ResponsibleParty>
          </gmd:distributorContact>
          <gmd:distributionOrderProcess>
            <gmd:MD_StandardOrderProcess>
              <gmd:fees>
                <gco:CharacterString>In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.</gco:CharacterString>
              </gmd:fees>
              <gmd:orderingInstructions>
                <gco:CharacterString>Contact NCEI for other distribution options and instructions.</gco:CharacterString>
              </gmd:orderingInstructions>
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          </gmd:distributionOrderProcess>
          <gmd:distributorFormat>
            <gmd:MD_Format>
              <gmd:name>
                <gco:CharacterString>JPEG</gco:CharacterString>
              </gmd:name>
              <gmd:version gco:nilReason="unknown" />
            </gmd:MD_Format>
          </gmd:distributorFormat>
          <gmd:distributorFormat>
            <gmd:MD_Format>
              <gmd:name>
                <gco:CharacterString>CSV</gco:CharacterString>
              </gmd:name>
              <gmd:version gco:nilReason="unknown" />
            </gmd:MD_Format>
          </gmd:distributorFormat>
          <gmd:distributorFormat>
            <gmd:MD_Format>
              <gmd:name>
                <gco:CharacterString>Shapefile</gco:CharacterString>
              </gmd:name>
              <gmd:version gco:nilReason="unknown" />
            </gmd:MD_Format>
          </gmd:distributorFormat>
          <gmd:distributorTransferOptions>
            <gmd:MD_DigitalTransferOptions>
              <gmd:transferSize>
                <gco:Real>1.28</gco:Real>
              </gmd:transferSize>
              <gmd:onLine>
                <gmd:CI_OnlineResource>
                  <gmd:linkage>
                    <gmd:URL>https://www.ncei.noaa.gov/archive/accession/0247568</gmd:URL>
                  </gmd:linkage>
                  <gmd:protocol>
                    <gco:CharacterString>HTTPS</gco:CharacterString>
                  </gmd:protocol>
                  <gmd:applicationProfile>
                    <gco:CharacterString>Web browser</gco:CharacterString>
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                  <gmd:name>
                    <gco:CharacterString>NCEI Dataset Landing Page</gco:CharacterString>
                  </gmd:name>
                  <gmd:description>
                    <gco:CharacterString>Navigate directly to the URL for a descriptive web page with download links.</gco:CharacterString>
                  </gmd:description>
                  <gmd:function>
                    <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
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                </gmd:CI_OnlineResource>
              </gmd:onLine>
              <gmd:onLine>
                <gmd:CI_OnlineResource>
                  <gmd:linkage>
                    <gmd:URL>https://www.ncei.noaa.gov/archive/accession/oas/247568</gmd:URL>
                  </gmd:linkage>
                  <gmd:protocol>
                    <gco:CharacterString>HTTPS</gco:CharacterString>
                  </gmd:protocol>
                  <gmd:applicationProfile>
                    <gco:CharacterString>Web browser</gco:CharacterString>
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                  <gmd:name>
                    <gco:CharacterString>Descriptive Information</gco:CharacterString>
                  </gmd:name>
                  <gmd:description>
                    <gco:CharacterString>Navigate directly to the URL for a descriptive web page with download links.</gco:CharacterString>
                  </gmd:description>
                  <gmd:function>
                    <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
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                </gmd:CI_OnlineResource>
              </gmd:onLine>
              <gmd:onLine>
                <gmd:CI_OnlineResource>
                  <gmd:linkage>
                    <gmd:URL>https://www.ncei.noaa.gov/archive/accession/download/247568</gmd:URL>
                  </gmd:linkage>
                  <gmd:protocol>
                    <gco:CharacterString>HTTPS</gco:CharacterString>
                  </gmd:protocol>
                  <gmd:applicationProfile>
                    <gco:CharacterString>Web browser</gco:CharacterString>
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                  <gmd:name>
                    <gco:CharacterString>HTTPS</gco:CharacterString>
                  </gmd:name>
                  <gmd:description>
                    <gco:CharacterString>Navigate directly to the URL for data access and direct download.</gco:CharacterString>
                  </gmd:description>
                  <gmd:function>
                    <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnLineFunctionCode>
                  </gmd:function>
                </gmd:CI_OnlineResource>
              </gmd:onLine>
              <gmd:onLine>
                <gmd:CI_OnlineResource>
                  <gmd:linkage>
                    <gmd:URL>ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0197/0247568/</gmd:URL>
                  </gmd:linkage>
                  <gmd:protocol>
                    <gco:CharacterString>FTP</gco:CharacterString>
                  </gmd:protocol>
                  <gmd:applicationProfile>
                    <gco:CharacterString>Any FTP client</gco:CharacterString>
                  </gmd:applicationProfile>
                  <gmd:name>
                    <gco:CharacterString>FTP</gco:CharacterString>
                  </gmd:name>
                  <gmd:description>
                    <gco:CharacterString>These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).</gco:CharacterString>
                  </gmd:description>
                  <gmd:function>
                    <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnLineFunctionCode>
                  </gmd:function>
                </gmd:CI_OnlineResource>
              </gmd:onLine>
            </gmd:MD_DigitalTransferOptions>
          </gmd:distributorTransferOptions>
        </gmd:MD_Distributor>
      </gmd:distributor>
    </gmd:MD_Distribution>
  </gmd:distributionInfo>
  <gmd:dataQualityInfo>
    <gmd:DQ_DataQuality>
      <gmd:scope>
        <gmd:DQ_Scope>
          <gmd:level>
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        </gmd:DQ_Scope>
      </gmd:scope>
      <gmd:lineage>
        <gmd:LI_Lineage>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>NCEI Accession 0247568 v1.1 was published.</gco:CharacterString>
              </gmd:description>
              <gmd:dateTime>
                <gco:DateTime>2022-03-21T16:13:15Z</gco:DateTime>
              </gmd:dateTime>
              <gmi:output>
                <gmi:LE_Source>
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                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>NCEI Accession 0247568 v1.1</gco:CharacterString>
                      </gmd:title>
                      <gmd:date>
                        <gmd:CI_Date>
                          <gmd:date gco:nilReason="inapplicable" />
                          <gmd:dateType>
                            <gmd:CI_DateTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_DateTypeCode" codeListValue="publication">publication</gmd:CI_DateTypeCode>
                          </gmd:dateType>
                        </gmd:CI_Date>
                      </gmd:date>
                      <gmd:citedResponsibleParty>
                        <gmd:CI_ResponsibleParty>
                          <gmd:individualName gco:nilReason="inapplicable" />
                          <gmd:contactInfo>
                            <gmd:CI_Contact>
                              <gmd:onlineResource>
                                <gmd:CI_OnlineResource>
                                  <gmd:linkage>
                                    <gmd:URL>https://www.ncei.noaa.gov/archive/accession/0247568/1.1</gmd:URL>
                                  </gmd:linkage>
                                  <gmd:protocol>
                                    <gco:CharacterString>HTTPS</gco:CharacterString>
                                  </gmd:protocol>
                                  <gmd:name>
                                    <gco:CharacterString>NCEI Accession 0247568 v1.1</gco:CharacterString>
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                                  <gmd:description>
                                    <gco:CharacterString>published 2022-03-21T16:13:15Z</gco:CharacterString>
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                                    <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnLineFunctionCode>
                                  </gmd:function>
                                </gmd:CI_OnlineResource>
                              </gmd:onlineResource>
                            </gmd:CI_Contact>
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                          <gmd:role gco:nilReason="inapplicable" />
                        </gmd:CI_ResponsibleParty>
                      </gmd:citedResponsibleParty>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
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      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:dataQualityInfo>
    <gmd:DQ_DataQuality>
      <gmd:scope>
        <gmd:DQ_Scope>
          <gmd:level>
            <gmd:MD_ScopeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
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      </gmd:scope>
      <gmd:lineage>
        <gmd:LI_Lineage>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: ZOOPLANKTON BIOMASS (measured);
Units: milligrams per cubic meter;
Observation Category: model output;
Sampling Instrument: net - zooplankton net;
Sampling and Analyzing Method: 0.5 or 1 meter diameter, 153 µm mesh net, conical, 2m-long;
Data Quality Method: Counts and identifications were cross checked between Cavaletto and Agy.</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: ZOOPLANKTON DENSITIES (measured);
Units: number per cubic meter;
Observation Category: laboratory analysis;
Sampling Instrument: net - zooplankton net;
Sampling and Analyzing Method: 0.5 or 1 meter diameter, 153 µm mesh net, conical, 2m-long;
Data Quality Method: Counts and identifications were cross checked between Cavaletto and Agy</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
          <gmd:organisationName>
            <gco:CharacterString>NOAA National Centers for Environmental Information</gco:CharacterString>
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          <gmd:role>
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        </gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation id="AMS">
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be32">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>microscope</gco:CharacterString>
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          <gmi:type>
            <gco:CharacterString>microscope</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>instrument is used in lab analyses

A microscope is an instrument used to see objects that are too small for the naked eye.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_beab">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>net - zooplankton net</gco:CharacterString>
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          <gmi:type>
            <gco:CharacterString>net - zooplankton net</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>net - zooplankton net</gco:CharacterString>
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      <gmi:platform>
        <gmi:MI_Platform id="plat_b185">
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            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>LAURENTIAN</gco:CharacterString>
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          </gmi:identifier>
          <gmi:description>
            <gco:CharacterString>LAURENTIAN</gco:CharacterString>
          </gmi:description>
          <gmi:instrument gco:nilReason="missing" />
        </gmi:MI_Platform>
      </gmi:platform>
      <gmi:platform>
        <gmi:MI_Platform id="plat_b3bd">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>SHENEHON</gco:CharacterString>
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            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:description>
            <gco:CharacterString>SHENEHON</gco:CharacterString>
          </gmi:description>
          <gmi:instrument gco:nilReason="missing" />
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      </gmi:platform>
      <gmi:platform>
        <gmi:MI_Platform id="plat_8b9e">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>USEPA Lake Guardian</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:description>
            <gco:CharacterString>IMO number = 8030609
Launch = 1981
reference = https://www.epa.gov/great-lakes-monitoring/lake-guardian</gco:CharacterString>
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          <gmi:instrument gco:nilReason="missing" />
        </gmi:MI_Platform>
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</gmi:MI_Metadata>