gov.noaa.nodc:0242879 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2025-09-26T19:30:38Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Differential proteomic analysis of the massive star coral Orbicella faveolata exposed to experimentally elevated temperatures (NCEI Accession 0242879) 2021-12-06 publication 2021-11-08 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0242879 NCEI Archive Management System 0242879 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Anderson B. Mayfield NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) andersonblairmayfield@gmail.com https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information resourceProvider NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information resourceProvider Anderson Mayfield NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) andersonblairmayfield@gmail.com https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information principalInvestigator Mayfield, Anderson B. NOAA Atlantic Oceanographic and Meteorological Laboratory https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information author NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information contributor tableDigital An inshore-to-offshore gradient in reef coral resilience to high temperatures exists in the Upper Florida Keys, with massive corals of the highly turbid nearshore reefs demonstrating higher capacity to resist summer bleaching than offshore conspecifics. After corroborating this trend with diverse Orbicella faveolata genotypes exposed to elevated temperatures in the laboratory, a proteomic analysis was taken with a subset of 20 samples via iTRAQ labeling followed by nano-liquid chromatography+mass spectrometry; 46 host coral and 40 Symbiodiniaceae dinoflagellate proteins passed all stringent quality control criteria, and the partial proteomes of biopsies of 1) healthy controls, 2) sub-lethally-stressed samples, and 3) actively bleaching corals differed significantly from one another. The proteomic data were then used to train predictive models of coral colony bleaching susceptibility, and both generalized multivariate regression and neural network models were capable of doing so with 100% accuracy. Successful future testing of the predictive power of these models in situ will establish the capacity to monitor coral health on a proactive, pre-death timescale. These proteomic (i.e., protein concentration) data were collected in order to better understand the molecular basis of resilience to rising seawater temperatures in replicate genotypes of the framework-building Caribbean coral Orbicella faveolata collected from several reef sites within the upper Florida Keys (see appended documents for permitting information, coordinates, and other supporting meta-data). Funding Agency: National Oceanic and Atmospheric Administration completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0242879 Preview graphic PNG 0242879 NCEI ACCESSION NUMBER 2021-10-23 publication biological data CORAL theme NODC DATA TYPES THESAURUS mass spectrometer instrument NODC INSTRUMENT TYPES THESAURUS biological laboratory analyses model output theme NODC OBSERVATION TYPES THESAURUS NOAA Atlantic Oceanographic and Meteorological Laboratory dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS NOAA Atlantic Oceanographic and Meteorological Laboratory dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS CORAL REEF STUDIES project NODC PROJECT NAMES THESAURUS Coastal Waters of Florida Florida Keys National Marine Sanctuary North Atlantic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DOC/NOAA/OAR/AOML > Atlantic Oceanographic and Meteorological Laboratory, OAR, NOAA, U.S. Department of Commerce dataCentre Global Change Master Directory (GCMD) Data Center Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Omics-NRDD18978 project Provider Project Names Genetic Data Sets > Proteomic Analysis theme CoRIS Discovery Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals EARTH SCIENCE > Biosphere > Zoology > Corals > Coral Diseases > Bleaching > Bleaching Thermal Stress EARTH SCIENCE > Biosphere > Zoology > Corals > Photic Zone Corals EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Proteomic Analysis EARTH SCIENCE > Biosphere > Zoology > Corals > Scleractinia (stony corals) theme CoRIS Theme Thesaurus EARTH SCIENCE > BIOLOGICAL CLASSIFICATION EARTH SCIENCE > SOLID EARTH > GEOMORPHIC LANDFORMS/PROCESSES > COASTAL LANDFORMS > CORAL REEFS theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Orbicella faveolata predictive model protein concentration protein sequences theme Provider Keywords MASS SPECTROMETERS > MASS SPECTROMETERS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Q Exactive mass spectrometer instrument Provider Instruments GBN1 platform Provider Platform Names COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Cheeca Rocks (24N080W0009) COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Conch Reef (24N080W0011) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Cheeca Rocks (24N080W0009) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Conch Reef (24N080W0011) place CoRIS Place Thesaurus OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF AMERICA OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Little Conch The Rocks place Provider Place Names 8EHUTJ Send2NCEI Submission Package ID accessLevel: Public otherRestrictions Cite as: Mayfield, Anderson B. (2021). Differential proteomic analysis of the massive star coral Orbicella faveolata exposed to experimentally elevated temperatures (NCEI Accession 0242879). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0242879. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Differential proteomics of the massive Caribbean coral Orbicella faveolata. Diversity, In Review 2021-11-30 publication Mayfield, Anderson B. author MDPI publisher documentDigital Diversity In Review crossReference sciencePaper Mayfield, A. (2020). MassIVE MSV000086530 - Towards predicting the fate of reef corals [Data set]. MassIVE. https://doi.org/10.25345/c55z0q 2020 publication International DOI Foundation (IDF) https://doi.org/10.25345/c55z0q https://doi.org/10.25345/c55z0q HTTPS https://doi.org/10.25345/c55z0q Protein sequences, analysis, and code from this study. This repository is typically used by the proteomics community and can house relevant code that can not be archived at NCEI information Mayfield, Anderson author MassIVE publisher tableDigital crossReference collection Mayfield, Anderson (2021). Shotgun proteomics of thermally challenged Caribbean reef corals (NCEI Accession 0227133). NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0227133. 2021-07-30 publication NOAA National Centers for Environmental Information gov.noaa.nodc:0227133 https://www.ncei.noaa.gov/archive/accession/0227133 HTTPS https://www.ncei.noaa.gov/archive/accession/0227133 Related samples and analysis information Mayfield, Anderson Cooperative Institute for Marine and Atmospheric Studies https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference collection eng; USA utf8 environment oceans biota -80.61573 -80.50205 24.89742 24.95375 2017-06-25 2017-12-14 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. CSV Excel Word fasta raw mzML mzid 3202.452 https://www.ncei.noaa.gov/archive/accession/0242879 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/242879 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/242879 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0191/0242879/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0242879 v1.1 was published. 2021-12-06T21:45:08Z NCEI Accession 0242879 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0242879/1.1 HTTPS NCEI Accession 0242879 v1.1 published 2021-12-06T21:45:08Z download dataset Parameter or Variable: protein concentration (measured); Units: ratio to control sample; Observation Category: laboratory analysis; Sampling Instrument: Q Exactive mass spectrometer; Sampling and Analyzing Method: Corals were sampled from multiple reefs in the Florida Keys National Marine Sanctuary, cored, and used in laboratory experiments. Upon sampling, proteins were extracted, labeled using iTRAQ technology (Sciex, USA) and analyzed via mass spectrometry. Please see the accompanying documents for methodological details.; Data Quality Method: Proteins were quantified using a standard BCA assay and assessed for quality via gel electrophoresis (SDS-PAGE; see accompanying Word documents for details.). Parameter or Variable: protein sequences (measured); Units: amino acids; Observation Category: laboratory analysis; Sampling Instrument: Q Exactive mass spectrometer; Sampling and Analyzing Method: Please see documents "Mayfield OFAV iTRAQ methods" and "Mayfield OFAV iTRAQ appendix" for details.; Data Quality Method: Please see documents "Mayfield OFAV iTRAQ methods" and "Mayfield OFAV iTRAQ appendix" for details. Parameter or Variable: predictive model (calculated); Units: coral bleaching susceptibility (probability); Observation Category: model output; Sampling Instrument: not applicable; Sampling and Analyzing Method: Please see documents "Mayfield OFAV iTRAQ methods" and "Mayfield OFAV iTRAQ appendix" for details.; Data Quality Method: Please see documents "Mayfield OFAV iTRAQ methods" and "Mayfield OFAV iTRAQ appendix" for details. asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian mass spectrometer mass spectrometer Instrument that measure the massess and relative concentrations of atoms and molecules