gov.noaa.nodc:0242497 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-03-24T18:44:01Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Water column and sediment geochemical data taken by bottle and bottom samples from NOAA Ship Ronald H. Brown in the southeastern U.S. Atlantic margin from 2019-04-10 to 2019-04-29 (NCEI Accession 0242497) 2021-11-01 publication 2021-10-29 creation NOAA National Centers for Environmental Information gov.noaa.nodc:0242497 NCEI Archive Management System 0242497 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Hannah Choi University of Georgia (UGA) hannahlychoi@uga.edu https://uga.edu/ HTTPS University of Georgia (UGA) website Institution web page information resourceProvider University of Georgia, School of Marine Programs http://www.marsci.uga.edu HTTP University of Georgia, School of Marine Programs website Institution web page information resourceProvider Samantha Joye University of Georgia, School of Marine Programs mjoye@uga.edu http://www.marsci.uga.edu HTTP University of Georgia, School of Marine Programs website Institution web page information principalInvestigator Kimberley Hunter University of Georgia, School of Marine Programs kshunter@uga.edu http://www.marsci.uga.edu HTTP University of Georgia, School of Marine Programs website Institution web page information collaborator Hunter, Kimberley author Choi, Hannah University of Georgia (UGA) hannahlychoi@uga.edu https://uga.edu/ HTTPS website Institution web page information author Joye, Samantha author University of Georgia, School of Marine Programs http://www.marsci.uga.edu HTTP University of Georgia, School of Marine Programs website Institution web page information contributor tableDigital This dataset contains water column and sediment geochemical data collected taken by bottle and bottom samples from NOAA Ship Ronald H. Brown (cruise RB19-03) in the southeastern U.S. Atlantic margin in April 2019. Data include nutrient, nitrate, phosphorus, and other chemical characteristics. There are CTD data for this expedition already archived on NCEI under accession number 0207828. This cruise is part of the DEEP SEARCH project, the goal of which is to explore a variety of deep sea ecosystems off the southeastern U.S. Atlantic margin. Data are in CSV format. This dataset is available to the public for a wide variety of uses including scientific research and analysis. Funding Agency: NOAA Ocean Exploration Funding Agency: US DOI; Bureau of Ocean Energy Management completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0242497 Preview graphic PNG 0242497 NCEI ACCESSION NUMBER 2021-10-26 publication AMMONIUM (NH4) CORE - POROSITY DELTA CARBON-13 DEPTH - BOTTOM DISSOLVED INORGANIC CARBON (DIC) DISSOLVED ORGANIC CARBON Dissolved Organic Nitrogen DISSOLVED OXYGEN LATITUDE LONGITUDE Methane (CH4) NITRATE nitrate + nitrite content (concentration) NITRITE PARTICULATE ORGANIC CARBON pH phosphate redox potential (Eh) SALINITY SEDIMENT - HYDROGEN SULPHIDE CONTENT SEDIMENT - IRON CONTENT SEDIMENTS - TOTAL CARBON Sulfate (SO4 2-) total alkalinity Total Dissolved Nitrogen (TDN) theme NODC DATA TYPES THESAURUS bottle chromatograph CTD laboratory analysis multibeam sonar Niskin bottle scintillation counter spectrophotometer Total Organic Carbon (TOC) analyzer instrument NODC INSTRUMENT TYPES THESAURUS chemical in situ laboratory analyses physical pore water chemistry sediment analysis theme NODC OBSERVATION TYPES THESAURUS NOAA Ship Ronald H. Brown platform NODC PLATFORM NAMES THESAURUS University of Georgia, School of Marine Programs dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS University of Georgia, School of Marine Programs dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS North Atlantic Ocean place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication DEEP Sea Exploration to Advance Research on Coral/Canyon/Cold seep Habitats (DEEP SEARCH) project Provider Projects EARTH SCIENCE > OCEANS > BATHYMETRY/SEAFLOOR TOPOGRAPHY > WATER DEPTH EARTH SCIENCE > OCEANS > MARINE SEDIMENTS EARTH SCIENCE > OCEANS > MARINE SEDIMENTS > SEDIMENT CHEMISTRY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRITE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITROGEN EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PHOSPHATE EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Aerobic Methane Oxidation (water column) Anarobic Methane Oxidation Rate (sediments) DISSOLVED INORGANIC CARBON (DIC) (pore water) DISSOLVED OXYGEN (water column) Dissolved Inorganic Nitrogen (DIN) Dissolved Inorganic Nitrogen to Dissolved Inorganic Phosphorus ratio (DIN:DIP) Dissolved Organic Nitrogen to Dissolved Organic Phosphorus ratio (DON:DOP) Dissolved Organic Phosphorus (DOP) Experimental anaerobic methane oxidation rate (sediments) Hydrogen (sediments) Hydrogen Sulfide (H2S) (pore water) Iron II oxide (pore water) Maximum section depth (sediments) Mean delta 13C of methane Minimum section depth (sediments) PARTICULATE ORGANIC CARBON (sediments) Redox potential (Eh) (sediments) Section midpoint depth (sediments) Sediment organic matter (loss on ignition method) Standard deviation delta 13C of methane Sulfate (SO4 2-) (pore water) Sulfate Reduction Rate (sediments) Total Dissolved Phosphorus Total Particulate Nitrogen (sediments) Total alkalinity (pore water) Total particulate phosphate (sediments) theme Provider Variable Names CTD > Conductivity, Temperature, Depth GAS CHROMATOGRAPHS > GAS CHROMATOGRAPHS MBES > Multibeam Mapping System NISKIN BOTTLES OPTSPEC > Optical Spectrometer SCINTILLATION COUNTERS > SCINTILLATION COUNTERS TOC > Total Organic Carbon Analyzer instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Antek Instruments 7050 nitric oxide (NO) chemiluminescent detector Beckmann Coulter LS6500 Liquid Scintillation Counter Dionex Integrion HPIC System Eh probe Hand Held Refractometer Peak Performer 1 gas chromatograph (GC) with reducing compound photometer (RCP) detector Picarro G2201-i Cavity Ring-Down Spectroscopy (CRDS) Ross pH electrode SRI 8610 Gas Chromatograph GC-FID Shimadzu TOC-V CPH Total Nitrogen Measurement (TNM) Total Nitrogen Analyzer Shimadzu UV1601 Spectrophotometer Thermo Orion dissolved oxygen (DO) probe ThermoFinnigan Flash EA 1112 series NC Soil Analyzer instrument Provider Instruments Ships platform Global Change Master Directory (GCMD) Platform Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ronald H. Brown (call sign: WTEC, ICES code: 33RO, 1997-) platform ICES/SeaDataNet Ship Codes Remotely Operated Vehicle (ROV) Jason platform Provider Platforms OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian U.S. Atlantic margin place Provider Place Names 6P8MJG Send2NCEI Submission Package ID accessLevel: Public otherRestrictions Cite as: Hunter, Kimberley; Choi, Hannah; Joye, Samantha (2021). Water column and sediment geochemical data taken by bottle and bottom samples from NOAA Ship Ronald H. Brown in the southeastern U.S. Atlantic margin from 2019-04-10 to 2019-04-29 (NCEI Accession 0242497). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0242497. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. Lunden, Jay; Cordes, Erik (2019). Water temperature, conductivity, salinity, and calculated sound velocity taken by CTD from NOAA Ship Ronald H. Brown in the North Atlantic Ocean from 2019-04-09 to 2019-04-29 (NCEI Accession 0207828). NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0207828. 2019-12-11 publication NOAA National Centers for Environmental Information gov.noaa.nodc:0207828 https://www.ncei.noaa.gov/archive/accession/0207828 HTTPS https://www.ncei.noaa.gov/archive/accession/0207828 Related dataset information Lunden, Jay Temple University (TU) jlunden@temple.edu http://www.temple.edu/ HTTP Temple University (TU) website Institution web page information author Cordes, Erik author NOAA National Centers for Environmental Information (NCEI) publisher tableDigital crossReference collection eng; USA utf8 environment oceans -79.25 -74.75 31.25 36 2019-04-10 2019-04-29 NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov pointOfContact In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media. Contact NCEI for other distribution options and instructions. Originator data format 0.344 https://www.ncei.noaa.gov/archive/accession/0242497 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/242497 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/242497 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0189/0242497/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0242497 v1.1 was published. 2021-11-01T20:04:11Z NCEI Accession 0242497 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0242497/1.1 HTTPS NCEI Accession 0242497 v1.1 published 2021-11-01T20:04:11Z download dataset Parameter or Variable: pH (measured); Units: pH scale; Observation Category: laboratory analysis; Sampling Instrument: Ross pH electrode; Sampling and Analyzing Method: Whole sediment pH was mesured using a sediment probe using a sediment probe (Oakton, Prod. No. 35634-50) during core sectioning. Pore water was extracted from sediments using a manual press. Measurements on small pore water volumes carried out using a flow-through cell. Ross electrode (Thermo Fisher, Prod. No. 8103BNUWP) was calibrated with buffers of pH 4, 7, and 10. Parameter or Variable: SALINITY (measured); Units: psu; Observation Category: laboratory analysis; Sampling Instrument: handheld refractometer Parameter or Variable: NITRITE (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: Shimadzu UV1601; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Pore water and water column samples were filtered through a pre-rinsed 0.2 micron cellulose filter and frozen until time of analysis. Nitrite was measured colorimetrically using method of Bendschneider and Robinson, 1952; reproduced by Parsons et al. 1984. Absorbance was measured on a Shimadzu UV1601 spectrophotometer. Parameter or Variable: nitrate + nitrite content (concentration) (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD, Antek Instruments 7050 NO detector; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Pore water and water column samples were filtered through a pre-rinsed 0.2 micron cellulose filter and frozen until time of analysis. NOx (nitrite + nitrate) was chemically reduced and measured with a nitric oxide detector according to the method of Garside 1981 using an Antek Instruments 7050 Nitric Oxide Detector with 745 Nitrate/Nitrite Reduction Assembly. Parameter or Variable: NITRATE (calculated); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD; Sampling and Analyzing Method: Calculated by subtracted measured nitrite concentration from measured NOx (nitrite + nitrate) concentration Parameter or Variable: AMMONIUM (NH4) (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD, Shimadzu UV1601; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Water column and pore water samples were filtered through a pre-rinsed 0.2 micron cellulose filter and frozen until time of analysis. Ammonium measured colorimetrically using the phenolhypochlorite method developed by Solorzano, 1969, on a Hach Company DR 2800 spectrophotometer. Parameter or Variable: Dissolved Inorganic Nitrogen (DIN) (calculated); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD; Sampling and Analyzing Method: Calculated by adding measured NOx (nitrite + nitrate) concentration and measured NH4 concentration Parameter or Variable: Total Dissolved Nitrogen (TDN) (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD, Shimadzu TOC-Vcph TNM Total Nitrogen Analyzer; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Water column and pore water samples were filtered through a pre-rinsed 0.2 micron cellulose filter and frozen until time of analysis. Samples analyzed using a Shimadzu Instruments TOC-Vcph Total Organic Carbon Analyzer with ASI-V Autosampler and TNM Total Nitrogen Analyzer, according to method of Watanabe et al. 2007. Parameter or Variable: Dissolved Organic Nitrogen (calculated); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD; Sampling and Analyzing Method: DON concentrations calculated by subtracted the calculated dissolved inorganic nitrogen concentration from the total dissolved nitrogen concentration Parameter or Variable: phosphate (measured); Units: micrmole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD, Shimadzu UV1601; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Water column and pore water samples were filtered through a pre-rinsed 0.2 micron cellulose filter and frozen until time of analysis. Phosphate was measured on a Shimadzu UV1601 spectrophotometer according to the colorimetric method of Strickland and Parsons, 1972. Parameter or Variable: Total Dissolved Phosphorus (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD, Shimadzu UV1601; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Water column and pore water samples were filtered through a 0.2 micron cellulose filter and frozen until time of analysis. TDP concentrations measured on a Shimadzu UV1601 spectrophotometer according to the colorimetric method of of Solorzano and Sharp, 1980. Parameter or Variable: Dissolved Organic Phosphorus (calculated); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: CTD; Sampling and Analyzing Method: Calculated by subtracting the measured phosphate concentrations from the measured total dissolved phosphorus concentrations Parameter or Variable: DIN:DIP (calculated); Units: molar ratio (unitless); Observation Category: laboratory analysis; Sampling Instrument: CTD; Sampling and Analyzing Method: Calculated by dividing the calculated DIN concentration by the measured phosphate concentration. Parameter or Variable: DON:DOP (calculated); Units: molar ratio (unitless); Observation Category: laboratory analysis; Sampling Instrument: CTD; Sampling and Analyzing Method: Calculated by dividing the calculated dissolved organic nitrogen concentration by the calculated dissolved organic phosphorus ratio. Parameter or Variable: DISSOLVED ORGANIC CARBON (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: Shimadzu TOC-Vcph TOC Analyzer; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Water column and pore water samples were filtered through a pre-rinsed 0.2 micron cellulose filter and frozen until time of analysis. DOC was measured on a Shimadzu Instruments TOC-Vcph Total Organic Carbon Analyzer with ASI-V Autosampler and TNM Total Nitrogen Analyzer according to the method of Sugimura and Suzuki, 1988. Parameter or Variable: LATITUDE (measured); Units: decimal degrees; Observation Category: other; Sampling Instrument: shipboard GPS Parameter or Variable: LONGITUDE (measured); Units: decimal degrees; Observation Category: other; Sampling Instrument: shipboard GPS Parameter or Variable: Sample depth (measured); Units: meters; Observation Category: in situ; Sampling Instrument: CTD Parameter or Variable: DEPTH - BOTTOM (measured); Units: meters; Observation Category: in situ; Sampling Instrument: multibeam sonar Parameter or Variable: Date (measured); Units: day/mo/yr; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: local time Parameter or Variable: Local time (measured); Units: hh:mm; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: Time of sample collection in Eastern Daylight Time (UTC-4:00) Parameter or Variable: redox potential (Eh) (sediments) (measured); Units: mV; Observation Category: laboratory analysis; Sampling Instrument: Eh probe; Sampling and Analyzing Method: Whole sediment Eh was measured during core sectioning. Parameter or Variable: minimum section depth (sediments) (measured); Units: centimeters below surface (cmbsf); Observation Category: laboratory analysis; Sampling Instrument: ruler; Sampling and Analyzing Method: The distance between sediment-water interface and the top of the sediment core section. Parameter or Variable: maximum section depth (sediments) (measured); Units: centimeters below surface (cmbsf); Observation Category: laboratory analysis; Sampling Instrument: ruler; Sampling and Analyzing Method: The distance between the sediment-water interface and the bottom of the sediment core section. Parameter or Variable: section midpoint depth (sediments) (calculated); Units: centimeters below surface (cmbsf); Observation Category: laboratory analysis; Sampling Instrument: ruler; Sampling and Analyzing Method: The distance between the sediment-water interface and the midpoint of the sediment core section, or, the average of the section minimum depth and section maximum depth Parameter or Variable: total alkalinity (pore water) (measured); Units: millimoles/liter; Observation Category: laboratory analysis; Sampling Instrument: Shimadzu UV1601 spectrophotometer; Sampling and Analyzing Method: Pore water was extracted using a manual press. Water was filtered through a 0.2 micron pre-rinsed cellulose filter. Alkalinity was determined using the spectrophotometric method described by Sarazin et al., 1999. Parameter or Variable: DISSOLVED INORGANIC CARBON (DIC) (pore water) (calculated); Units: millimoles/liter; Observation Category: laboratory analysis; Sampling Instrument: calculated; Sampling and Analyzing Method: DIC concentrations were calculated from the measured alkalinity values as follows: DIC = Alkalinity * (alpha1 + 2*alpha2) where alpha1 = [HCO3] * [DIC] alpha2 = [CO3] * [DIC] alpha1 and alpha2 were determined using K1 and K2 of carbonic acid dissolution pK1 and pK2 were determined from temperature and salinity according to Luecker et al. 2001: pK1 = 3633.86/T – 61.2172 + 9.67770 * ln(T) – 0.011555 * S + 0.0001152 * S2 pK2 = 471.178/T + 25.9290 – 3.1697 * ln(T) – 0.01781 * S + 0.0001122 * S2 Parameter or Variable: Methane (CH4) (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: SRI 8610 GC-FID; Sampling and Analyzing Method: Sediment samples (3 cubic centimeters) were collected into a glass serum vial, preserved with N2-purged 2M NaOH (3 milliliters), crimp-sealed with a butyl rubber stopper and stored at room temperature until analysis. Concentrations were determined by headspace analysis using an SRI 8610C gas chromatograph equipped with a flame ionization detector and SRI Hayesep D 6’x1/8” column (Prod. No. 8600-PKDB). Water column gas samples were collected by overflowing a 1 L PETG bottle and closing headspace-free with a silicone septum cap. A modified 1 L glass media bottle was evacuated to a pressure of 0.2 Torr or below. A 0.7 L aliquot of sample from the PETG was pulled into the evacuated bottle. The 0.7 L sample was shaken and sonicated 3x before the pressure was equilibrated by the addition of brine purged with UHP nitrogen. Extracted gases were collected from the top of the modified media bottle using a syringe and stored in 20 mL serum vials filled with brine. Methane concentrations were determined by injecting gas samples from the brine vials onto an SRI 8610 GC-FID and SRI Hayesep D 6’x1/8” column (Prod. No. 8600-PKDB). See Schmitt, 1991 and Lammers, 1994. Parameter or Variable: Mean delta 13C of methane (measured); Units: permil; Observation Category: laboratory analysis; Sampling Instrument: Picarro G2201-i CRDS; Sampling and Analyzing Method: Methane isotopic ratios were measured out of the same vials as methane concentrations. 2 mL (sediments) or 5 ml (water column) of headspace from the methane vials (3 cc sediment preserved with 3 mL UHP N2 purged 2 M NaOH for sediments, 20 mL vials of brine for water column) was injected into the Small Sample Introduction Module of the Picarro CRDS. Parameter or Variable: Standard deviation delta 13C of methane (measured); Units: permil; Observation Category: laboratory analysis; Sampling Instrument: Picarro G2201-i CRDS; Sampling and Analyzing Method: Standard deviation of several measurements taken over the course of 4 minutes by the Picarro G2201-i CRDS. Parameter or Variable: Hydrogen (sediments) (measured); Units: nanomoles/liter; Observation Category: laboratory analysis; Sampling Instrument: Peak Performer 1 GC with RCP detector; Sampling and Analyzing Method: Sediment (5 cc) was collected into a 20 mL headspace vial and sealed with a blue butyl chloride stopper and aluminum crimp seal. Vials were purged with nitrogen gas for ~5 minutes, then stored at 4 C for 4-5 days. The headspace was analyzed for hydrogen using a Peak Laboratories Peak Performer 1 gas chromatograph with RCP detector. Parameter or Variable: Hydrogen Sulfide (H2S) (pore water) (measured); Units: millimoles/liter; Observation Category: laboratory analysis; Sampling Instrument: Shimadzu UV1601 spectrophotometer; Sampling and Analyzing Method: Pore water was extracted using a manual press. 2 mL unfiltered porewater was collected into a 15 mL centrifuge tube containing 100 uL or 500 uL 20% zinc acetate w/v solution and stored at 5°C until analysis. H2S was determined using the colorimetric method described by Cline, 1969. Parameter or Variable: Sulfate (SO4 2-) (pore water) (measured); Units: millimoles/liter; Observation Category: laboratory analysis; Sampling Instrument: Dionex Integrion HPIC; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Water was filtered through a pre-rinsed 0.2 micron cellulose filter. Samples were collected in 7 ml glass vials and preserved with 10 ul HNO3 per ml of sample. Samples were diluted for analysis on a Dionex Integrion HPIC with AS-AP autosampler, AS19 guard and analytical columns,; Data Quality Method: Data were corrected for analytical bias and for evaporation using the ratio of measured chloride concentration to expected chloride concentration. Parameter or Variable: Iron II (pore water) (measured); Units: micromole/liter; Observation Category: laboratory analysis; Sampling Instrument: Shimadzu UV1601 spectrophotometer; Sampling and Analyzing Method: Pore water was extracted from sediments using a manual press. Pore water was filtered through a pre-rinsed 0.2 micron cellulose filter. Samples were collected in 7 ml glass vials and preserved with 10 ul HNO3 per ml of sample. Iron (II) was measured using the spectrophotometric method described by Stookey, 1970. Parameter or Variable: Anarobic Methane Oxidation Rate (sediments) (measured); Units: nanomoles per cubic centimeter per day; Observation Category: laboratory analysis; Sampling Instrument: Beckmann-Coulter LS6500; Sampling and Analyzing Method: Live sediment (3 cc) was transferred into a modified Hungate tube with a Hungate stopper and cap on one end and a modified, movable blue butyl chloride stopper at the other end. Three replicates were sampled per depth, with one kill control sampled per core. Kill controls were killed using 2M NaOH. UHP methane was added to samples expected to have high in situ methane concentrations for a target concentration of 15 mM. Samples were injected with 100 microliters of 14C-methane tracer (1 million dpm). Samples were incubated at in situ pressure and temperature for 1-3 days before termination by addition of 3 mL 2 M NaOH. Samples were later transferred to Erlenmeyer flasks, acidified with 5 mL 30% sulfuric acid, and distilled for 6-8 hours. Evolved carbon dioxide was captured using 3-methoxypropylamine and counted on a Beckman-Coulter 6500 liquid scintillation counter. Rates were calculated using the ratio of counted 14C carbon dioxide to the injected tracer activity and the measured in situ methane concentration. Method described in Bowles et al. 2011 and Joye et al. 2004, adapted from Iversen and Blackburn 1981. Parameter or Variable: Experimental anaerobic methane oxidation rate (sediments) (measured); Units: nanomoles per cubic centimeter per day; Observation Category: laboratory analysis; Sampling Instrument: Beckmann-Coulter LS6500; Sampling and Analyzing Method: Method the same as for Anaerobic Methane Oxidation Rate, except that the added methane (15 mM) plus in situ methane concentration was used in the rate calculation. Parameter or Variable: Sulfate Reduction Rate (sediments) (measured); Units: nanomoles per cubic centimeter per day; Observation Category: laboratory analysis; Sampling Instrument: Beckmann-Coulter LS6500; Sampling and Analyzing Method: Live sediment (3 cc) was transferred into a modified Hungate tube with a Hungate stopper and cap on one end and a modified, movable blue butyl chloride stopper at the other end. Three replicates were sampled per depth, with one kill control sampled per core. Kill controls were killed using 3 mL 20% zinc acetate. Samples were injected with 100 microliters of 35S sodium sulfate tracer (10 million dpm). Samples were incubated at in situ pressure and temperature for 1-3 days before termination by addition of 3 mL 20% zinc acetate. Samples were later spin down in a centrifuge at 5000 rpm for 2 min twice. The supernatant was subsampled and counted to determine the activity of injected sulfate. Sediments were then distilled in hot hydrochloric acid and reduced chromium chloride (TRIS distillations). Evolved hydrogen sulfide was captured in zinc acetate traps and counted on a Beckman-Coulter 6500 liquid scintillation counter. Rates were calculated using the ratio of captured 35S sulfide to injected sulfate activity, the measured pore water sulfate concentration, and the sediment porosity. Method described in Bowles et al. 2011, and Joye et al. 2004, adapted from Fossing and Jorgensen 1989. Parameter or Variable: CORE - POROSITY (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: analytical balance; Sampling and Analyzing Method: Sediment samples were collected into glass scintillation vials, capped and stored at 4°C until analysis. Porosity was determined by drying a sample of known mass at 80°C, weighing, and calculating the moisture content. Parameter or Variable: Sediment organic matter - loss on ignition (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: analytical balance; Sampling and Analyzing Method: Sediment samples were collected into glass scintillation vials, capped and stored at 4°C until analysis. SOM was determined by drying a sample at 80°C, weighing, ashing at 500°C, weighing and calculating weight lost on ignition. Parameter or Variable: Total Particulate Nitrogen (sediments) (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: ThermoFinnigan FlashEA 1112 series NC Soil Analyzer; Sampling and Analyzing Method: A pressed sediment sample (mud-cake, removed from pore water press after extraction) was dried at 60°C and then homogenized via grinding. TPN was determined by the method described by Gordan, Jr., 1969 as reproduced by Sharp, 1974. Samples were analyzed on a ThermoFinnigan FlashEA 1112 series NC Soil Analyzer. Parameter or Variable: CARBON - TOTAL PARTICULATE - SEDIMENT (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: Thermofinnigan FlashEA 1112 series NC Soil Analyzer; Sampling and Analyzing Method: A pressed sediment sample (mud-cake, removed from pore water press after extraction) was dried at 60°C and then homogenized via grinding. TPC was determined by the method described by Gordan, Jr., 1969 as reproduced by Sharp, 1974. Samples were analyzed on a ThermoFinnigan FlashEA 1112 series NC Soil Analyzer. Parameter or Variable: PARTICULATE ORGANIC CARBON (sediments) (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: ThermoFinnigan FlashEA 1112 series NC Soil Analyzer; Sampling and Analyzing Method: A pressed sediment sample (mud-cake, removed from pore water press after extraction) was acidified with 1 N HCl, dried at 60°C, and then homogenized via grinding. POC was determined by the method described by Gordan, Jr., 1969 as reproduced by Sharp, 1974. Samples were analyzed on a ThermoFinnigan FlashEA 1112 series NC Soil Analyzer. Parameter or Variable: Total particulate phosphate (sediments) (measured); Units: micromole/gram sediment; Observation Category: laboratory analysis; Sampling Instrument: Shimadzu UV1601 spectrophotometer; Sampling and Analyzing Method: A pressed sediment sample (mud-cake, removed from pore water press after extraction) was dried at 80°C, ground, and distributed into 20 mL scintillation vials. The samples were prepared using the method described by Zhou et. al., 2003. TPP and TPP percent were determined using the colormetric method described by Solorzano and Sharp, 1980. Parameter or Variable: DISSOLVED OXYGEN (water column) (measured); Units: milligrams/liter; Observation Category: laboratory analysis; Sampling Instrument: Thermo Orion DO probe; Sampling and Analyzing Method: DO was measured with a Thermo Orion DO probe (Prod. No. 083005MD) while sample was constantly stirred. Parameter or Variable: Collection Type (measured); Units: none; Observation Category: other; Sampling Instrument: CTD, ROV; Sampling and Analyzing Method: identifies whether the sample was collected using CTD Niskin or ROV Niskin Parameter or Variable: Zulu time (measured); Units: hh:mm; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: Time of sample collection in UTC Parameter or Variable: Aerobic Methane Oxidation (water column) (measured); Units: picomoles/liter/day; Observation Category: laboratory analysis; Sampling Instrument: Beckmann Coulter LS6500; Sampling and Analyzing Method: Four technical replicates were collected from each sampled depth by filling 16.6 mL Hungate tubes headspace-free and sealing with exetainer septa. Tubes were stored at 4 °C until injection. One of the four replicates was killed by displacing 1.6 ml sample with 1.6 ml 37% formalin. All samples, including the killed abiotic control, were injected with ~2000000 dpm tritiated methane tracer. Samples were incubated at near-in situ temperature for 1-4 days. Immediately before incubation termination, a 100 ul subsample was removed from each tube and measured on a liquid scintillation counter to determine total dissolved 3H-methane addition. Incubations were terminated by killing with 1.6 ml 37% formalin and purging with air for 1 hour to remove remaining tracer. A 5 ml aliquot of each killed replicate was measured on a liquid scintillation counter to determine the ratio of tracer turnover. The turnover ratio was multiplied by the measured in situ methane concentration to obtain the MOx rate. Killed controls were subtracted and the replicates averaged to obtain the final rate number. See Rogener, 2018 asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian bottle bottle any type of water bottle sampling device Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle. chromatograph chromatograph Gas, ion, liquid or other chromatograph CTD CTD Conductivity-Temperature-Depth probe laboratory analysis laboratory analysis use as an observation type multibeam sonar multibeam sonar generic instrument to measure seafloor topography Active sensors that utilize acoustic energy to collect measurements of seafloor depth and character (from http://www.csc.noaa.gov/crs/rs_apps/sensors/multi_beam.htm (last accessed 2008-12-09). Seafloor topography, bathymetry and other related geophysical data are usually managed and archived at the NOAA National Geophysical Data Center (www.ngdc.noaa.gov/mgg/). Niskin bottle Niskin bottle Device for obtaining samples of seawater at a specific depth Why special entry for Niskin bottle but no entry for Nansen bottle? scintillation counter scintillation counter measures ionizing radiation A scintillation counter is an instrument for detecting and measuring ionizing radiation by using the excitation effect of incident radiation on a scintillating material, and detecting the resultant light pulses. spectrophotometer spectrophotometer spectrophotometer, optical spectrometer, spectrograph or spectroscope An optical spectrometer (spectrophotometer, spectrograph or spectroscope) is an instrument used to measure properties of light over a specific portion of the electromagnetic spectrum, typically used in spectroscopic analysis to identify materials. Butler, L. R. P.; Laqua, K. (1995). "Nomenclature, symbols, units and their usage in spectrochemical analysis-IX. Instrumentation for the spectral dispersion and isolation of optical radiation (IUPAC Recommendations 1995)". Pure Appl. Chem. IUPAC. 67 (10): 1725–1744. doi:10.1351/pac199567101725. A spectrometer is the general term for describing a combination of spectral apparatus with one or more detectors to measure the intensity of one or more spectral bands." Total Organic Carbon (TOC) analyzer Total Organic Carbon (TOC) analyzer measures the total amount of organic carbon contained in water NOAA Ship Ronald H. Brown Updated information from ICES for UNITED STATES platform Ronald H. Brown Flag : UNITED STATES - US (ISO Country Code) Call Sign : WTEC Title : NOAA Ship Platform Class : Research vessel IMO : 9105786 Pennant : R 104 Commissioned Date : 1997-07-19 Current Length : 83.5 Built : 1997 Information Source : http://www.moc.noaa.gov/rb/index.html (URL) Lloyds URL : http://www.seasearcher.com/lmiu/vessels/overview.htm?vesselID=277516 *General Comments:* Name RESEARCHER II planned during build was never used.