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          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/project/details/727" xlink:actuate="onRequest">Ocean Carbon and Acidification Data System (OCADS)</gmx:Anchor>
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          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/project/details/675" xlink:actuate="onRequest">US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)</gmx:Anchor>
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          <gmd:keyword>
            <gmx:Anchor xlink:href="https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/seaname/details/91" xlink:actuate="onRequest">North Atlantic Ocean</gmx:Anchor>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="place">place</gmd:MD_KeywordTypeCode>
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                <gco:CharacterString>Ocean Acidification Search Keywords</gco:CharacterString>
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          <gmd:keyword>
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          <gmd:keyword>
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            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="theme">theme</gmd:MD_KeywordTypeCode>
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                    <gco:Date>2026</gco:Date>
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                            <gco:CharacterString>HTTPS</gco:CharacterString>
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                <gco:CharacterString>OCADS Study Type</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Dissolved Inorganic Carbon;
Abbreviation: DIC;
Unit: umol/kg;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Discrete dissolved inorganic carbon (DIC) measurement (Non-dispersive infrared, or NDIR);
Analyzing instrument: Apollo SciTech;
Detailed sampling and analyzing information: DIC samples were analyzed immediately upon collection without the addition of preservatives. Samples were collected at time zero and at 48 hours. Mean of values is reported .;
CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography.;
CRM batch number: 175;
Preservation method: not preserved;
Preservative volume: none;
Preservative correction: none;
Uncertainty: 2.7 µmol/kg;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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pH scale: seawater scale;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Measured;
Sampling instrument: Spectrophotometer;
Analyzing instrument: Cary 100;
Detailed sampling and analyzing information: eawater samples were collected from each beaker (time=0 and 48 hrs) during the experiment, and analyzed for pH using a 10 cm cells. Mean values are reported of the two measurements.;
Standardization description: TRIS from Andrew Dickson lab was used;
Standardization frequency: daily;
At what temperature was pH reported: 20;
Uncertainty: ± 0.0014;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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Unit: Month-Day-Year;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: in-situ observation;
Measured or calculated: Measured;
Duration: 48 hour after exposure;
Detailed sampling and analyzing information: Date of experiment;
Uncertainty: zero;
Biological subject: black sea bass;
Species ID: 167687</gco:CharacterString>
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Abbreviation: Salinity;
Unit: pratical salinity;
Observation type: Laboratory experiment;
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Measured or calculated: Measured;
Sampling instrument: YSI - handheld multi-parameter instrument;
Duration: 48 hours;
Detailed sampling and analyzing information: YSI used to measure salinity;
Biological subject: Black Sea bass;
Species ID: 159348;
Researcher name: Shannon Meseck;
Researcher institution: NOAA NMFS</gco:CharacterString>
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Abbreviation: pCO2 out;
Unit: uatm;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Calculated;
Calculation method and parameters: CO2SYS;
Duration: 48 hours;
Detailed sampling and analyzing information: DIC and pH values were entered into CO2SYS (Pierrot et al., 2006) to calculate ρCO2 (µatm), Ωcalcite, and Ωaragonite using the following constants: the constants: K1, K2 from Lueker et al. (2000); K hydrogen sulfate from Dickson (1990); and total Boron from Lee et al. (2010) . Reported at 20 C for temperature and pressure of 0. Nutrients were at zero for input.;
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Species ID: 159348</gco:CharacterString>
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                <gco:CharacterString>Parameter or Variable: Omega Calcite;
Abbreviation: Calcite;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: manipulation condition;
Measured or calculated: Calculated;
Calculation method and parameters: CO2SYS;
Duration: 48 hours;
Detailed sampling and analyzing information: DIC and pH values were entered into CO2SYS (Pierrot et al., 2006) to calculate ρCO2 (µatm), Ωcalcite, and Ωaragonite using the following constants: the constants: K1, K2 from Lueker et al. (2000); K hydrogen sulfate from Dickson (1990); and total Boron from Lee et al. (2010) . Reported at 20 C for temperature and pressure of 0. Nutrients were at zero for input.;
Biological subject: Black Sea bass;
Species ID: 159348</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Hatch (%);
Abbreviation: Hatch;
Unit: %;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Calculated;
Sampling instrument: Microscope;
Duration: 48 hours;
Detailed sampling and analyzing information: Samples were taken after 48 hours exposure. Samples were preserved in 10% buffered formalin. Samples were enumerated on a microscope then the % hatch was calculated. The percentage hatch was % of hatchlings divided by total stocking density of fertilized eggs multiplied by 00;
Replicate information: all samples counted 4 times;
Biological subject: black sea bass;
Species ID: 159348;
Researcher name: Dylan Redman;
Researcher institution: NOAA NMFS</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: Abnormal (%);
Abbreviation: Abnormal;
Unit: %;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Calculated;
Sampling instrument: Microscope;
Duration: 48 hours;
Detailed sampling and analyzing information: Samples were taken after 48 hours exposure. Samples were preserved in 10% buffered formalin. Samples were analyzed on a microscope where the spine was identified as either normal (straight) or abnormal (curved). The number of abnormal hatchlings divided by total number of hatchlings times 100 for a percentage.;
Replicate information: each sample counted 4 times.;
Biological subject: black sea bass;
Species ID: 159348;
Researcher name: Dylan Redman;
Researcher institution: NOAA</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
          <gmd:processStep>
            <gmi:LE_ProcessStep>
              <gmd:description>
                <gco:CharacterString>Parameter or Variable: % not hatched;
Abbreviation: % not hatched;
Unit: %;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: response variable;
Measured or calculated: Measured;
Sampling instrument: Microscope;
Analyzing instrument: Microscope;
Duration: 48 hours;
Detailed sampling and analyzing information: The number of embryos that did not hatch after 48 hours. This was the total live embryos unhatched divided by total stocking density multiplied by 100%.;
Replicate information: 4;
Biological subject: Black sea Bass;
Species ID: 159348</gco:CharacterString>
              </gmd:description>
            </gmi:LE_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
          <gmd:organisationName>
            <gco:CharacterString>NOAA National Centers for Environmental Information</gco:CharacterString>
          </gmd:organisationName>
          <gmd:role>
            <gmd:CI_RoleCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="custodian">custodian</gmd:CI_RoleCode>
          </gmd:role>
        </gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation id="AMS">
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be32">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>microscope</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>microscope</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>instrument is used in lab analyses

A microscope is an instrument used to see objects that are too small for the naked eye.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_bef3">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>multi-parameter water quality sensor</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>multi-parameter water quality sensor</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>used to measure temperature, salinity, dissolved oxygen, and other parameters

Instrument used to measure temperature, salinity, dissolved oxygen, and other parameters, often used to measure water quality parameters. Can be a hand-held instrument or a sonde for unattended monitoring. Often used in coastal water monitoring stations. YSI is the preeminent brand (as of 2024).</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be24">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>Non-dispersive infrared (NDIR) dissolved inorganic carbon (DIC) analyzer</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Non-dispersive infrared (NDIR) dissolved inorganic carbon (DIC) analyzer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>A known amount of seawater is dispensed into a stripping chamber where it is acidified and purged with an inert gas. The amount of CO2 in the resulting gas stream is determined with a non-dispersive infrared (NDIR) carbon dioxide analyzer.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument id="inst_be73">
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gco:CharacterString>spectrophotometer</gco:CharacterString>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>spectrophotometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>spectrophotometer, optical spectrometer, spectrograph or spectroscope

An optical spectrometer (spectrophotometer, spectrograph or spectroscope) is an instrument used to measure properties of light over a specific portion of the electromagnetic spectrum, typically used in spectroscopic analysis to identify materials.

Butler, L. R. P.; Laqua, K. (1995). "Nomenclature, symbols, units and their usage in spectrochemical analysis-IX. Instrumentation for the spectral dispersion and isolation of optical radiation (IUPAC Recommendations 1995)". Pure Appl. Chem. IUPAC. 67 (10): 1725–1744. doi:10.1351/pac199567101725. A spectrometer is the general term for describing a combination of spectral apparatus with one or more detectors to measure the intensity of one or more spectral bands."</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
    </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>