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https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Upper%20thermal%20tolerance%20in%20red%20and%20blue%20king%20crab%3B%20Sublethal%20and%20lethal%20effects |
https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Long%3B%20Data%20from%3B%20Upper%20thermal%20tolerance%20in%20red%20and%20blue%20king%20crab%3B%20Sublethal%20and%20lethal%20effects |
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/16/2021.
Contact: Daniel.woodrich@noaa.gov |
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https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords |
GCMD Keyword Forum Page |
Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt,
MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard
Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA).
URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords |
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https://www.afsc.noaa.gov |
Website |
Website for this organization |
information |
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https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center |
Alaska Fisheries Science Center Website |
AKFSC Home Page |
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https://www.fisheries.noaa.gov/inport/item/35877 |
Full Metadata Record |
View the complete metadata record on InPort for more information about this dataset. |
information |
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https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/35877.pdf |
NOAA Data Management Plan (DMP) |
NOAA Data Management Plan for this record on InPort. |
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https://www.westcoast.fisheries.noaa.gov/ |
West Coast Regional Office Website |
Website listed for West Coast Regional Office |
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Initially, seven different temperatures were tested for both species: 14, 16, 18,
20, 22, 24, 26°C. As the trials were performed, we added an additional temperature
treatment for each species at around the estimated LT50 ( temperature that causes
50% mortality): 21°C for blue king crab and 25°C for red king crab. The trials were
run in a random order. For the seven temperatures tested in common among the species,
both species were tested simultaneously. For each trial, 14 year-0 red and blue king
crab were haphazardly assigned to each treatment. At the beginning of the experiments,
all the tanks were at ambient temperature (i.e., the same temperature the crabs were
being held at). The temperature in each tank was then increased gradually to the experimental
temperature over 4 hours. The crabs were left at the temperature for 24 h after which
they were checked for activity by gently prodding them with a dissecting needle and
observing whether they responded by moving away from the needle. They were then checked
to see if they were alive.
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In this experiment, the effects of temperature on feeding ration were determined for
red and blue king crabs. Temperatures tested included 11, 13, 15, 17, 20, and 23°C
for red king crab and 4, 6, 9, 11, 13, 15, 17, and 20°C for blue king crab.Year-1
blue king crab and year-0 red king crab were used. In each trial 5 (blue king crab)
or 7 (red king crab) crab were massed and then introduced into individual holding
cells. The temperature was slowly adjusted to the treatment temperature over 4 h.
Crab were allowed to rest for 24h to acclimate them to the temperature and to standardize
hunger levels. Then crab were fed small pieces of massed squid mantle and allowed
to feed for 24h. At the same time, pieces of food were also placed in three control
cells without crab. After this, the remaining food was removed and massed. Daily feeding
ration, expressed as a percentage of the crab?s mass, was calculated for each crab
and corrected for the change of mass of the food in the control cells assuming a minimum
feeding ration of 0%. In a few cases, crab molted during the trial or ate all the
food and the data from these crab was excluded from analysis.
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To determine how temperature affects growth and long-term survival in year-0 crabs
we performed 45 day experiments. Red king crab were tested at 12, 15, and 20°C and
blue king crab at 12, 15, and 17°C. Twelve crabs per species per treatment were haphazardly
selected. Crabs had their carapace width measured and were introduced into individual
holding cells. The temperature was slowly adjusted to the treatment temperature over
4 h. Crabs were checked daily for molting or mortality and were fed to excess. After
the end of the experiment each crab had their carapace width measured a second time.
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