| 1 |
|
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Pathobiology%3B%202014%20Bitter%20crab%20disease%20prevalence%20in%20immature%20Chionoecetes%20spp.%20at%206%20index%20sites%20in%20eastern%20Bering%20Sea.csv |
https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/SAP/Pathobiology%3B%202014%20Bitter%20crab%20disease%20prevalence%20in%20immature%20Chionoecetes%20spp.%20at%206%20index%20sites%20in%20eastern%20Bering%20Sea.csv |
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/15/2021.
Contact: Daniel.woodrich@noaa.gov |
download |
| 1 |
|
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords |
GCMD Keyword Forum Page |
Global Change Master Directory (GCMD). 2026. GCMD Keywords, Version 24. Greenbelt,
MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard
Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA).
URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords |
information |
| 1 |
|
https://www.afsc.noaa.gov |
Website |
Website for this organization |
information |
| 1 |
|
https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center |
Alaska Fisheries Science Center Website |
AKFSC Home Page |
information |
| 1 |
|
https://www.fisheries.noaa.gov/inport/item/26477 |
Full Metadata Record |
View the complete metadata record on InPort for more information about this dataset. |
information |
| 1 |
|
|
This dataset contains randomly chosen samples of crab collected from the annual NOAA/NMFS/AFSC/RACE
crab-groundfish bottom trawl survey of the eastern Bering Sea continental shelf. The
standard survey area, surveyed each year since 1975, encompasses a major portion of
the eastern Bering Sea shelf between the 20 meter and 200 meter isobaths and from
the Alaska Peninsula to the north of St. Matthew Island. The study area is divided
into a grid with cell sizes of 20 x 20 nautical miles (37 x 37 kilometers). Sampling
takes place within each 20 x 20 nautical mile grid cell. Each tow is one-half hour
in duration, averaging 1.54 nautical miles (2.86 kilometers) - exact tow duration
and distance fished for each haul can be found in RACEBASE.HAUL.
|
| 1 |
|
|
Six specific index sites were chosen (three C. bairdi sites and three C. opilio sites)
based on historical incidences of the parasite Hematodinium sp. the causative agent
of Bitter Crab Syndrome (BCS). Index sites are located in Bristol Bay, Pribilof Islands,
St. Matthew Island and the northern shelf edge (within stratum 61) and northern stations
(within stratum 82) and each site included approximately 10 survey stations. At each
station 20 immature crab were randomly selected, if less than 20 were available, additional
crab were selected at another station within the index site. Whole crab or legs from
crab were preserved by freezing and shipped frozen to Seattle (AFSC).
|
| 1 |
|
|
The sex of the crab is identified (1 = Male, 2 = Female, 3 = Unknown). Individual
crab carapaces were measured (± 1 mm), excluding spines, and are reported as size.
Null or blank fields in data represent no size information reported for crab specimen.
Shell condition class serves as a semi-quantitative index of molt status and time
in shell post-molt. Carapace shell condition was assigned to one of six classes according
to specific criteria (0 = premolt or molting, 1 = soft and pliable, 2 = new hardshell
both firm and clean, 3 = oldshell slightly worn, 4 = oldshell worn, 5 = very oldshell,
9 = no shell condition information reported for crab specimen).
|
| 1 |
|
|
All female crab abdomens were evaluated to determine reproductive condition based
on the color of the eggs (0 = no eggs, 2 =purple, 3 = brown, 4 = orange, 5 = purple-brown,
6 = pink, 9 = no egg color information reported or does not apply), the condition
of the eggs (0 = no eggs, 1 = uneyed, 2 = eyed, 3 = dead, 4 = empty egg cases, 9 =
no egg condition information reported or does not apply), and the size of the egg
clutch (0 = immature, 1 = mature female no eggs, 2 = trace to 1/8, 3 = 1/4, 4 = 1/2,
5 = 3/4, 6 = full, 9 = no egg clutch information reported or does not apply).
|
| 1 |
|
|
Frozen tissue was removed from leg muscle and placed into 100% ethanol. DNA was extracted
using modified Ivanova et al. (2006) protocol.
Extracted DNA was assayed with two primer sets, targeting either 18S or ITS1 rDNA,
to determine presence or absence of Hematodinium spp. Refer to metadata Point of
Contact for PCR conditions. PCR Result is identified (0 = negative for Hematodinium
DNA, 1 = positive for Hematodinium DNA, 3 = undetermined, at the limit of detection).
|