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http://www.jstor.org/stable/pdf/20487736.pdf |
Citation URL |
Swiney, K. M. 2008. Egg extrusion, embryo development, timing and duration of eclosion,
and incubation period of primiparous and multiparous tanner crabs (Chionoecetes bairdi).
Journal of Crustacean Biology 28(2): 334-341 Manuscript produced from this data |
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http://www.jstor.org/stable/pdf/4126708.pdf?acceptTC=true |
Citation URL |
Stevens, B. G., and K. M. Swiney. 2007. Hatch timing, incubation period, and reproductive
cycle for captive primiparous and multiparous red king crab, Paralithodes camtschaticus.
Journal of Crustacean Biology 27(1): 37-48. Manuscript cited in the methods section |
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http://www.nrcresearchpress.com/doi/pdf/10.1139/z98-147 |
Citation URL |
Miriyasu M. and C. Lanteigne 1998. Embryo development and reproductive cycle in the
snow crab, Chionoecetes opilio (Crustacea: Majidae), in the southern Gulf of St. Lawrence,
Canada. Canadian Journal of Zoology 76: 2040-2048 Manuscript cited in the methods
section |
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https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Swiney%3B%20Primiparous%20and%20multiparous%20Tanner%20crab%20egg%20extrusion,%20embryo%20development%20and%20hatching |
https://console.cloud.google.com/storage/browser/nmfs_odp_afsc/RACE/SAP/Swiney%3B%20Primiparous%20and%20multiparous%20Tanner%20crab%20egg%20extrusion,%20embryo%20development%20and%20hatching |
Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/16/2021.
Contact: Daniel.woodrich@noaa.gov |
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https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords |
GCMD Keyword Forum Page |
Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt,
MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard
Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA).
URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords |
information |
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https://www.afsc.noaa.gov |
Website |
Website for this organization |
information |
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https://www.fisheries.noaa.gov/about/alaska-fisheries-science-center |
Alaska Fisheries Science Center Website |
AKFSC Home Page |
information |
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https://www.fisheries.noaa.gov/inport/item/25614 |
Full Metadata Record |
View the complete metadata record on InPort for more information about this dataset. |
information |
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https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/25614.pdf |
NOAA Data Management Plan (DMP) |
NOAA Data Management Plan for this record on InPort. |
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Female Tanner crabs were captured in the field November 2002 through May 2003 and
brought to the Kodiak Fisheries Research Center (KFRC) laboratory. Females were grouped
as either primiparous or multiparous. Females that comprised the multiparous group
were collected by commercial crab pots fished in Chiniak Bay, Kodiak, Alaska (57°43.25'
N, 152°17.5' W,), November, 2002 and delivered to the laboratory. The females collected
were ovigerous, hatched their larvae in the laboratory and extruded a new clutch of
eggs. Once a female began stripping her pleopods after eclosion, she was paired with
a mature male; therefore, the female could either mate or use stored sperm to fertilize
a new clutch. It is not know how many previous clutches females in the multiparous
group brooded, but they were multiparous because they were collected ovigerous and
then extruded a new clutch of eggs in the laboratory. Primiparous females were collected
by divers while still pubescent and nearing their terminal molt (as indicated by being
in a grasping pair) in Womens Bay, Kodiak, Alaska (57°43.6' N, 152°32.0' W, Fig. 2).
Divers searched for pubescent females in mating pairs from November 2002 through May
2003; however, females used in this study were collected between December 2002 and
February 2003, except for one female collected in April 2003. The grasping pairs were
isolated in underwater cages that were monitored regularly until a female underwent
her terminal molt, mated and extruded eggs after which time she was brought into the
laboratory. Occasionally, a female underwent her terminal molt in a cage without a
male present. When this occurred, the soft shelled female was brought into the laboratory
and mated with a male recently collected from the field.
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All females were tagged with an oval 6.5 mm × 20 mm individually numbered plastic
Floy tag attached with a cable tie to a fourth walking leg. Data recorded were female
reproductive state (primiparous or multiparous), molting and mating dates, dates of
egg extrusion and carapace width measured as the greatest straight-line distance across
the carapace excluding spines. Mean extrusion dates were calculated as the average
date of extrusion within the primiparous and multiparous groups.
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For the duration of the study, crabs were held in a single flow-through tank 0.46
m × 0.46 m × 1.8 m at the KFRC seawater laboratory. Seawater intakes are located at
15 and 26 m depths in Trident Basin approximately 30 m from the laboratory. Both primiparous
and multiparous females were held in the same tank to ensure that all crabs in the
experiment were exposed to identical conditions. Sand-filtered seawater was used and
the tank was chilled from May 2003 through November 2003 to ensure appropriate temperatures.
An Onset StowAway TidBiT data logger recorded water temperature in the tank. Crabs
were fed ad libitum twice weekly a diet of fish and squid.
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Twenty-four primiparous females and 21 multiparous females were used in this experiment;
however, crabs died over the course of the experiment, leaving 14 primiparous and
9 multiparous females at the end. Crabs were sampled on the 15th (± 2) day of every
month beginning in January 2003 and ending April 2004, when eclosion occurred. Upon
visual examination, all crabs and their eggs appeared healthy when they were sampled.
Females died over the course of the experiment, but there was no obvious reason to
eliminate the eggs of these females (collected before death) from the study since
the eggs appeared to be healthy and developing normally when collected.
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Monthly sampling was a two-step process in which embryo developmental stages were
determined and digital images of eggs were captured. A randomly sampled egg clump
was removed from each female with forceps and approximately 20 eggs were immersed
in Bouin?s solution for 5 minutes to facilitate observation of the external morphology
of the embryos (Moriyasu and Lanteigne, 1998). After embryos reached the eyed stage,
they were not placed in Bouin?s solution. Embryo developmental stages were determined
using a compound microscope at a total magnification of 50x and developmental stages
followed those of Moriyasu and Lanteigne (1998) for snow crabs, Chionoecetes opilio
(Fabricius, 1788). Additionally, digital images of 10 fresh eggs from each female
were taken with a digital camera attached to a compound microscope at a total magnification
of 50x; only fresh eggs were used because preserved eggs swell (Moriyasu and Lanteigne,
1998). Image analysis software (Image Pro Plus Version 4.1 for Windows, Media Cybernetics,
Inc. 8484 Georgia Avenue, Suite 200, Silver Spring, Maryland 20910) was used to measure
egg area rather than diameter to reduce measurement bias due to the imperfect sphericity
of the eggs.
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At the end of April 2004, females were placed in individual containers with flow-through
seawater at ambient temperature, and nets (101.5 mm with 350 micron mesh) were placed
on the seawater outflow to retain all the larvae. Newly hatched larvae were collected
daily from each female as in Stevens and Swiney (2007); the amount of larvae released
was determined by measuring volume. Larvae from each net were transferred to a graduated
cylinder and seawater was added; after the larvae settled, the volume (mL) of larvae
in the cylinder was recorded. For the purposes of analysis in this study, eclosion
began when 0.1 mL of larvae were collected and ended when females began stripping
their pleopods clean which coincided with the last of the larvae hatching. Mean hatch
date was the weighted average of larval output over time calculated by multiplying
the daily volume of hatched larvae by day-of-the-year, summing the products over time
and dividing by the total volume of larvae released. Incubation period was the number
of days between when a female extruded eggs and the last day of hatching.
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