Metadata Identifier: gov.noaa.nmfs.inport:12673

Aggregation Info | Bands | Citations | Constraints | Coverage Descriptions | Dimensions | Extents | Formats | Geographic Bounding Box
Georectified Information | Georeferenceable Information | Identifiers | Instruments | Mediums | OnlineResources | Operations
Platforms | Process Steps | Range Elements | Reference Systems | Responsible Parties | Series | Sources | Spatial Grids | Temporal Extents

MD_DataIdentification

Count Component Title Abstract
1 AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis Walleye pollock are an important component of the eastern Bering Sea ecosystem due to their vast numbers and biomass and are of great commercial importance. Their recruitment in the eastern Bering Sea is not well understood but it is largely determined during the first year, so the larval stage may play a significant role. Starvation may contribute to the high and variable mortality that occurs during the larval stage so it is important to monitor and quantify it. This metadata describes data from laboratory and field studies conducted from 2009 to 2012 for NPRB project 926. The project completed the development of an assay using flow cytometric cell cycle analysis to assess the physiological condition of walleye pollock larvae, and applied it to field collected specimens. The use of RNA to improve the accuracy of the assay was also investigated, as well as the relationship between flow cytometric measurements and growth. Results showed that the overall classification accuracy of the assay (healthy or unhealthy) ranged between 75 and 83% depending on the type of cross-validation testing. A nuclear RNA variable (the ratio of the number of S phase nuclei to the number of high nuclear RNA content G1 phase nuclei) improved overall classification accuracy by 11% compared to a model without it. Unhealthy walleye pollock larvae were present in the southeastern Bering Sea in 2009, and this was most likely the result of cold environmental conditions affecting prey availability and larval feeding. In 2010, no unhealthy larvae were detected. This may be the result of less spatial coverage and smaller collections of larvae than in 2009, given that the environmental conditions were similar between years.
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SV_Identification

none found
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CI_Citation

Count Component Title Date Citation Identifier
1 AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis

12673
1 No Title Global Change Master Directory (GCMD) Data Center Keywords
    2017-04-24
1 InPort
    1 NOAA Data Management Plan (DMP)
      12673
      2 NOAA/NMFS/EDM
        1 PARR Exclusion
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          CI_Series

          none found
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          CI_ResponsibleParty

          Count Component Individual Organization Position Email Role Linkage
          2 https://www.fisheries.noaa.gov/inport/item/12673
          1 Alaska Fisheries Science Center resourceProvider https://www.afsc.noaa.gov
          2 Bahl, Kimberly kimberly.bahl@noaa.gov pointOfContact
          1 GCMD Landing Page Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) custodian https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
          1 Porter, Steve steve.porter@noaa.gov custodian
          1 Porter, Steve steve.porter@noaa.gov distributor
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          CI_OnlineResource

          Count Component Linkage Name Description Function
          1 https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv https://console.cloud.google.com/storage/browser/_details/nmfs_odp_afsc/RACE/RPP/NPRB%20926%3B%20Results%20from%202009%20laboratory%20experiment%20measuring%20nuclear%20RNA.csv Note: Dataset migrated by Dan Woodrich (AFSC data management coordinator) on 12/14/2021. Contact: Daniel.woodrich@noaa.gov download
          1 https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 22. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information
          1 https://www.afsc.noaa.gov Website Website for this organization information
          1 https://www.fisheries.noaa.gov/inport/item/12673 Full Metadata Record View the complete metadata record on InPort for more information about this dataset. information
          1 https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/afsc/dmp/pdf/12673.pdf NOAA Data Management Plan (DMP) NOAA Data Management Plan for this record on InPort. information
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          MD_Identifier or RS_Identifier

          Count Component Code
          2 12673
          1 Bering Sea
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          EX_Extent

          Bounding Box Temporal Extent
          Count Component Description West East North South Start End
          1 -171.1 -163.3 57.4 54.3 2009 2012
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          EX_GeographicBoundingBox

          Count Component West East North South
          1 -171.1 -163.3 57.4 54.3
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          EX_TemporalExtent

          Count Component Start End
          1 2009 2012
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          MD_Format

          Count Component Name Version specification
          1 CSV - Comma Separated Values (Text)
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          MD_Medium

          none found
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          MD_Constraints

          Count Component Use Limitation
          1 NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
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          MD_ReferenceSystem

          none found
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          MD_GridSpatialRepresentation

          none found
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          MD_Georeferenceable or MI_Georeferenceable

          none found
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          MD_Georectified or MI_Georectified

          none found
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          MD_Dimension

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          MD_CoverageDescription or MI_CoverageDescription

          none found
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          MD_Band or MI_Band

          none found
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          MI_RangeElementDescription

          none found
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          MD_AggregateInformation

          Count Component Title Code Association Type Code
          1 NOAA Data Management Plan (DMP) crossReference
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          LE_Source or LI_Source

          none found
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          LE_ProcessStep or LI_ProcessStep

          none found
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          MI_Operation

          none found
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          MI_Platform

          none found
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          MI_Instrument

          none found
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